成功建立了仙人掌离体快繁的实验体系 ,并且对影响微繁殖的一些因素 ,诸如激素组合、外植体的物理状态、大量元素的含量等进行了研究。结果表明 :BA对仙人掌芽增殖具明显作用 ,MS +BA 5 .0mg/L +IBA 0 .1mg/L为最适增殖培养基 ;接种方式实验表明劈接优于整棵。钙、镁离子浓度对试管苗生长没有影响 ,但影响生根数及根长 ;NAA抑制根的伸长 ,但一定浓度可促进生根。总体而言 ,最适的生根培养基为 1 /2MS。同时发现块接比单芽接具有优势。试管苗在形态上出现一些变异。实验结果对仙人掌科其它植物的快速繁殖具有参考意义 Successfully established the experimental system of cactus in vitro propagation, and some factors that affect micropropagation, such as hormone combinations, the physical state of explants, the content of a large number of elements were studied. The results showed that: BA had a significant effect on the proliferation of cactus buds, with MS + BA 5.0 mg / L and IBA 0.1 mg / L as the optimal proliferation medium. The inoculation method showed that the split was better than the whole plant. Calcium and magnesium ion concentration had no effect on the growth of test tube seedlings, but the root number and root length were affected. NAA inhibited root elongation, but certain concentration could promote rooting. Overall, the most suitable rooting medium is 1 / 2MS. At the same time, it is found that the block connection has the advantage over the single bud connection. Test tube seedlings in the shape of some variation. The results of the experiment cactus rapid propagation of other plants with reference