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目的对细菌性感染新生儿的T细胞亚群CD3、CD4、CD8和中性粒细胞CD64的表达进行研究,探讨CD3、CD4、CD8和CD64在新生儿细菌感染诊断中的价值。方法选择2013年1月-2014年12月在汉川市人民医院儿科收治的细菌感染新生儿80例作为感染组,同期收治的无感染新生儿80例作为对照组,比较感染组和对照组新生儿白细胞计数、C-反应蛋白水平和CD64表达及CD3、CD4和CD8的表达情况,比较感染组新生儿治疗前后CD3、CD4、CD8和CD64表达的变化情况,分析CD3、CD4、CD8的表达和CD64表达的相关性。结果感染组新生儿C-反应蛋白和CD64表达均明显高于对照组新生儿(P<0.05),感染组新生儿白细胞计数和对照组比较没有差异(P>0.05)。感染组新生儿CD3、CD4的表达均明显低于对照组新生儿(P<0.05),感染组新生儿CD8表达和对照组比较没有差异(P>0.05)。感染组新生儿治疗后CD3和CD4表达高于治疗前(P<0.05);感染组新生儿治疗后CD64表达低于治疗前(P<0.05);感染组新生儿治疗前后CD8的表达情况没有明显变化(P>0.05)。CD3和CD64的表达呈负相关(P<0.05);CD4和CD64的表达呈负相关(P<0.05);CD8和CD64的表达无相关性(P>0.05)。结论新生儿细菌性感染患儿的CD64表达水平升高,CD3和CD4表达水平降低,细菌感染患儿存在一定的免疫功能异常,对细菌感染新生儿进行CD64、CD3和CD4表达水平的检测有利于疾病的早期诊断和治疗,并可以协助评估预后。
Objective To investigate the expression of CD3, CD4, CD8 and neutrophil CD64 in neonates with bacterial infection and to explore the value of CD3, CD4, CD8 and CD64 in the diagnosis of neonatal bacterial infection. Methods Eighty-eight neonates with bacterial infection were admitted to Hanchuan People’s Hospital from January 2013 to December 2014 as infection group, and 80 newborns with no infection at the same period were selected as the control group. The neonates of infection group and control group were compared. The levels of CD3, CD4, CD8, CD3, CD4 and CD8 in neonates before and after treatment were compared. The changes of CD3, CD4, CD8, CD64 expression. Results Neonatal C-reactive protein and CD64 expression in the infected group were significantly higher than those in the control group (P <0.05). There was no difference in neonatal white blood cell count between the infected group and the control group (P> 0.05). The expression of CD3 and CD4 in infected neonates was significantly lower than that in control neonates (P <0.05). There was no difference in neonatal CD8 expression between infected and control groups (P> 0.05). The expression of CD3 and CD4 in neonates after infection was higher than that before treatment (P <0.05). The expression of CD64 in neonates after infection was lower than that before treatment (P <0.05) Change (P> 0.05). There was a negative correlation between the expression of CD3 and CD64 (P <0.05). The expression of CD4 and CD64 was negatively correlated (P <0.05). There was no correlation between the expression of CD8 and CD64 (P> 0.05). Conclusions The level of CD64 and CD3 and CD4 in children with bacterial infection in neonates are higher than those in children with bacterial infections. The detection of CD64, CD3 and CD4 in neonates with bacterial infection is beneficial to the detection of CD64, CD3 and CD4 Early diagnosis and treatment of the disease, and can help assess the prognosis.