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应用反转录ＰＣＲ的技术从小鼠脾细胞中克隆了免疫共刺激信号Ｂ７－２编码区ｃＤ－ＮＡ。小鼠脾细胞经ＬＰＳ刺激２４ｈ后，提取总ＲＮＡ。以总ＲＮＡ为模板进小鼠Ｂ７－２ｃＤＮＡ的反转录ＰＣＲ扩增，获得一特异扩增带，与预期值相符。将扩增的ＤＮＡ克隆于ｐＵＣ１８质粒中，序列测定表明其序列与文献报道一致。
The co-stimulatory signal B7-2 coding region cD-NA was cloned from mouse spleen cells using reverse transcription PCR. Mouse spleen cells stimulated by LPS 24h, total RNA was extracted. Total RNA was used as a template to amplify the B7-2 cDNA in mice by reverse transcription PCR. A specific amplification band was obtained, which was consistent with the expected value. The amplified DNA was cloned into the pUC18 plasmid. Sequence analysis showed that the sequence was consistent with that reported in the literature.