目的 :研究高、低剪切力对动脉内皮通透性和紧密连接的影响。方法 :饲以普通和高脂肪食的两组日本大耳白兔的腹主动脉狭窄至原横断面积的 6 0 7%± 7 0 % ,7d后处死。动脉用艾万斯蓝和苏丹Ⅳ染色。紧密连接用冷冻蚀刻法检测。结果 :未狭窄腹主动脉染色阴性 ,紧邻狭窄近、远侧 1mm有恒定环行阳性区和距狭窄 8mm远侧内有散在点、片状阳性区。冷冻蚀刻显示在通透性增高区 ,狭窄远侧小带状型紧密连接的百分率明显低于近侧(P <0 0 1) ,远侧小带状和斑状型紧密连接的条数明显低于近侧 (P <0 0 1)。结论 :高、低剪切力导致内皮通透性增高及紧密连接变化 ,低剪切力区更易形成动脉硬化 Objective: To study the effect of high and low shear force on arterial endothelial permeability and tight junction. Methods: The abdominal aorta stenosis of two groups of Japanese white rabbits fed with normal and high-fat diet was 60.7% ± 70% of the original cross-sectional area, and then sacrificed 7 days later. Arteries were stained with Evans blue and Sudan IV. Closely linked by freeze-etching test. Results: The non-stenosed abdominal aorta was negatively stained, close to the stenosis, 1 mm distal to the positive circumscribed area and 7 mm distal to the stenosis were scattered, flaky positive area. Freeze-etching showed that in the hyperpermeability region, the percentage of tight junctions on the distal side of the stenosis was significantly lower than those on the proximal side (P <0.01), and the number of distal zonules and spots on the tight junctions was significantly lower Proximal (P <0 0 1). Conclusion: High and low shear force lead to increased endothelial permeability and tight junctional changes, and low shear stress zone is more likely to form arteriosclerosis