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目的 :探讨喉鳞状细胞癌 Hep- 2细胞系染色体畸变及其核型特征 ,认识喉癌的细胞遗传学改变与其发病机制的相关性。方法 :应用常规和高分辨 G显带方法进行核型分析。结果 :Hep- 2细胞系的染色体数目变化于 5 4～ 84条之间 ,众数为 6 9～ 74条。可识别其结构的稳定的标记染色体为 13条 ,部分核型中存在双微体 ,是基因扩增的细胞遗传学标志。结论 :喉癌 Hep- 2细胞系属超三倍体细胞 ,存在染色体数目和结构畸变并具有稳定的标记染色体及基因扩增。结构畸变涉及易位、缺失和等臂染色体。染色体断裂重接导致染色体重排 ,3、5、6和 8号染色体的缺失与重排是喉癌特征性改变
Objective: To investigate the chromosomal aberrations and karyotypes of laryngeal squamous cell carcinoma Hep-2 cell line and to find out the correlation between cytogenetic changes of laryngeal carcinoma and its pathogenesis. Methods: Karyotyping was performed using conventional and high-resolution G-banding methods. Results: The number of chromosomes in Hep-2 cell line varied from 54 to 84, and the number of chromosomes varied from 69 to 74. There are 13 stable marker chromosomes that can recognize its structure, and there are two micro-species in some karyotypes, which are the cytogenetics markers of gene amplification. CONCLUSION: Hep-2 cell line of laryngeal carcinoma belongs to the type of super-triploid cell with chromosome number and structure distortion, and has stable marker chromosome and gene amplification. Structural aberrations involve translocations, deletions and ischial chromosomes. Chromosomal rearrangements lead to chromosomal rearrangements, deletions and rearrangements of chromosomes 3, 5, 6 and 8 are characteristic changes in laryngeal cancer