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目的:探讨桑叶体外抗呼吸道合胞病毒(RSV)的作用。方法:采用细胞培养技术,以利巴韦林为阳性对照,观察比较桑叶对RSV引起的细胞病变(CPE)的抑制作用。结果:桑叶的半数中毒浓度(TC50)为21.98 mg/mL,预防给药方式、细胞外直接灭菌及治疗给药方式均有抗RSV作用,并存在量效关系;采用预防给药方式时,其抗RSV作用优于利巴韦林(P<0.05),IC50为0.38 mg/mL,TI为57.8;采用直接灭活、治疗给药方式时,其抗RSV作用与利巴韦林相当,IC50分别为0.77、1.25 mg/mL,TI分别为28.5、17.6。结论:桑叶对RSV有直接灭活作用,对RSV侵入Hela细胞有阻断作用,对RSV在Hela细胞内增殖有抑制作用。在相同质量浓度下,以预防作用更显著。
Objective: To investigate the anti-respiratory syncytial virus (RSV) effects of mulberry leaves in vitro. Methods: Using cell culture technique, ribavirin was used as a positive control to observe and compare the inhibitory effect of mulberry leaves on RSV-induced cytopathic effect (CPE). Results: The half poisoning concentration (TC50) of mulberry leaves was 21.98 mg/mL. The anti-RSV effect and the dose-effect relationship existed between the preventive administration, the extracellular direct sterilization and the therapeutic administration, and the dose-effect relationship was used. Its anti-RSV effect was superior to Ribavirin (P<0.05), IC50 was 0.38 mg/mL, and TI was 57.8. When direct inactivation and therapeutic administration were used, the anti-RSV effect was comparable to Ribavirin. IC50 were 0.77, 1.25 mg/mL, TI was 28.5, 17.6, respectively. Conclusion: Mulberry leaves have a direct effect on the inactivation of RSV, it has a blocking effect on RSV invading Hela cells, and has an inhibitory effect on the proliferation of RSV in Hela cells. At the same mass concentration, the preventive effect is more pronounced.