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目的探讨β2肾上腺素能受体(β2-AR)基因多态性对β2肾上腺素受体激动剂治疗婴幼儿毛细支气管炎疗效的影响。方法采用PCR扩增目的基因,以DNA直接测序法对β2肾上腺素能受体基因多态性进行分析,以喘憋、咳嗽、哮鸣音、啰音消失天数和住院天数评价β2肾上腺素受体激动剂的治疗效果。结果 (1)β2-AR 16位点多态性中Gly/Gly纯合子、Gly/Arg杂合子及Arg/Arg纯合子喘憋消失天数、咳嗽消失天数、哮鸣音消失天数、啰音消失天数和住院天数差异均有统计学意义(P<0.05)。(2)β2-AR 27位点不同基因型之间,喘憋消失天数、咳嗽消失天数、哮鸣音消失天数、啰音消失天数和住院天数未见明显差异。结论β2-AR 16位点多态性与β2肾上腺素受体激动剂治疗毛细支气管炎疗效具有相关性。
Objective To investigate the effect of β2-adrenergic receptor (β2-AR) gene polymorphism on β2-adrenergic receptor agonists in the treatment of infantile bronchiolitis. Methods The target gene was amplified by PCR and the β2 adrenergic receptor gene polymorphism was analyzed by DNA direct sequencing. The β2-adrenoceptor was evaluated in asthmatic rats, cough, wheezing, disappearing days and length of hospital stay Therapeutic effect of agonist. Results (1) The number of days of disappearance of the Gly / Gly homozygote, Gly / Arg heterozygote and Arg / Arg homozygote in the β2-AR 16 polymorphism, the number of cough disappearing days, the days of wheeze disappearing, the number of days of rales disappearing And hospitalization days were statistically significant (P <0.05). (2) There were no significant differences between different genotypes of β2-AR27, days of wheezing disappearing, days of cough disappearing, days of wheeze disappearing, days of rales disappearing and days of hospitalization. Conclusion The polymorphism of β2-AR 16 locus is related to the therapeutic effect of β2 adrenergic receptor agonists on bronchiolitis.