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采用高效液相色谱技术研究了苦皮藤素Ⅴ在粘虫Mythimna separata和小地老虎Agrotis ypsilon幼虫中的穿透及代谢。结果表明:苦皮藤素V均不能从粘虫和小地老虎幼虫的体壁穿透到血腔或从血腔穿透到中肠,但很容易从中肠穿透到血腔,且穿透速率无差异;苦皮藤素V在小地老虎幼虫体内的代谢解毒速率远大于其在粘虫幼虫体内的代谢速率,其半衰期分别为5.5和13.1 h。本研究结果表明,苦皮藤素V对粘虫和小地老虎幼虫的选择毒杀作用与药物的穿透能力无关,其在试虫体内的解毒代谢差异才是其对昆虫具有选择作用的机理之一。
The penetration and metabolism of celangulin Ⅴ in Mythimna separata and Agrotis ypsilon larvae were studied by high performance liquid chromatography (HPLC). The results showed that celangulin V could not penetrate from the body wall of the armyworm and the small Tigers larvae to the blood cavity or from the blood cavity to the midgut but could easily penetrate the midgut to the blood cavity and penetrate The rate of metabolism of celangulin V in the larvae of Landrace was far greater than that in the larvae of Mythimna separata with a half-life of 5.5 and 13.1 h, respectively. The results of this study indicate that the selective toxicity of celangulin V to armyworm and small tiger larvae has nothing to do with the penetration ability of the drug and the difference in detoxification metabolism in the test body is its mechanism of selective action on insects one.