以结球生菜马莱克（Ｌａｃｔｕｃａｓａｔｉｖａｃｖ．Ｍａｌｉｋａ）为试材，以ＭＳ为基本培养基，采用不同的激素配比，经愈伤组织诱导及芽分化、生根、移植入土三个步骤的离体培养，获得正常的再生植株，建立了结球生菜的基因转化受体系统，为下一步的基因转化工作提供了有利条件．愈伤组织诱导及芽分化培养基为ＭＳ＋ＢＡ０．１－４．０ｍｇ／Ｌ＋ＩＡＡ（或ＮＡＡ或２，４－Ｄ）０．０５－２．０ｍｇ／Ｌ；分根培养基为１／２ＭＳ．还测定了子叶外植体对抗生素的敏感性． Taking Lactucasativacv. Malika as the test material and MS as the basic medium, different hormonal ratios, callus induction and bud differentiation, rooting and transplantation were performed in three steps of in vitro culture, The normal regenerated plants were obtained, and the gene transformation receptor system was established for lettuce, which provided the favorable conditions for the next gene transformation. The callus induction and bud differentiation medium was MS + BA0.1-4.0mg / L + IAA (or NAA or 2,4-D) 0.05-2.0mg / L; rooting medium was 1 / 2MS. Cotyledon explants were also assayed for their antibiotic sensitivity.