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[Objective] To establish the methods for determining the contents of Mogroside V,Liquiritin and Glycyrrhizic acid in Luomai granule by HPLC.[Methods] The chromatographic conditions were as follows: the chromatographic column was ZORBAX SB-C18( 4. 6 mm× 250 mm,5 μm); the mobile phases were acetonitrile-water and 0. 1% phosphoric acid-acetonitrile respectively,and the flow rate was 0. 80 ml / min; the column temperature was 30 ℃,and the detection wavelength were 203 nm and 237 nm separately. [Results] The experiment proved that there was good linear relation when injection amount were in a range from 0. 95 μg to 4. 75 μg for Mogroside V( r = 0. 999 6),from0. 152 μg to 1. 064 μg for liquiritin( r = 0. 999 8) and from 0. 074 μg to 0. 518 μg from glycyrrhizic acid( r = 0. 999 8). The average recovery rate was 100. 06%,99. 96%,100. 08% respectively and the RSD were 1. 01%,1. 03%,1. 02%( n = 6) respectively.[Conclusions] This method is simple,accurate and highly reproducible,so it can be used to determine the contents of Mogroside V,Liquiritin and glycyrrhizic acid in Luomai granules,and can be applied to control the quality of Luomai granules.
[Objective] To establish the methods to determine the contents of Mogroside V, Liquiritin and Glycyrrhizic acid in Luomai granule by HPLC. [Methods] The chromatographic conditions were as follows: the chromatographic column was ZORBAX SB-C18 (4.6 mm × 250 the flow rate was 0.80 ml / min; the column temperature was 30 ° C, and the detection wavelength was 203 nm and 237 nm separately. [Results] The experiment proved that there was good linear relationship when injection amount were in a range from 0. 95 μg to 4. 75 μg for Mogroside V (r = 0. 999 6), from 0. 152 μg to 1.064 μg for liquiritin (r = 0.999 8) and from 0. 074 μg to 0. 518 μg from glycyrrhizic acid (r = 0.999 8). The average recovery rate was 100. 06%, 99. 01%, 1.03%, 1.02% (n = 6) respectively. [Conclusions] This method is simple, accurate and highly reproducible, so it c an be used to determine the contents of Mogroside V, Liquiritin and glycyrrhizic acid in Luomai granules, and can be applied to control the quality of Luomai granules.