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目的:探讨血小板源性生长因子(Platelet-derived growth factor,PDGF)DNA甲基化在同型半胱氨酸((homocysteine,Hcy)致血管平滑肌细胞(vascular smooth muscle cells,VSMCs)增殖的作用机制。方法:正常人脐动脉VSMCs体外培养,随机分为对照组、治疗组(Hcy100μmol/L+叶酸30μmol/L)、Hcy干预组(50、100、200、500μmol/L)总计6组,以MTT检测VSMCs的增殖率,巢式降落式PCR(nMS-PCR)法检测PDGF启动子区DNA甲基化的改变。结果:不同浓度Hcy干预的VSMCs与对照组和治疗组相比,随着Hcy浓度的增加VSMCs增殖率增加。治疗组与对照组比所有结果均无明显变化。PDGF甲基化水平增高,100μmol/L、200μmol/L Hcy组与对照组比较差异有统计学意义(P<0.01和P<0.05),而治疗组与对照组比较差异无统计学意义(P>0.05)。结论:Hcy可导致人VSMCs PDGF启动子区域甲基化异常从而促进VSMCs的增殖。
Objective: To investigate the mechanism of DNA methylation of platelet-derived growth factor (PDGF) in the proliferation of vascular smooth muscle cells (VSMCs) induced by homocysteine (Hcy). Methods: Normal human umbilical artery VSMCs were cultured in vitro and randomly divided into control group, Hcy treatment group (100μmol / L folic acid 30μmol / L) and Hcy intervention group (50,100,200,500μmol / L) (NMS-PCR) was used to detect the changes of DNA methylation in PDGF promoter region.Results: Compared with the control group and the treatment group, VSMCs treated with different concentrations of Hcy increased with increasing concentration of Hcy The proliferation rate of VSMCs was increased.The methylation level of PDGF was higher in the treatment group than in the control group, and there was significant difference between the 100μmol / L, 200μmol / L Hcy group and the control group (P <0.01 and P < 0.05), but there was no significant difference between the treatment group and the control group (P> 0.05) .Conclusion: Hcy can induce the hypermethylation of PDGF promoter region of human VSMCs and promote the proliferation of VSMCs.