目的　研究抑制胶原纤维酸性蛋白（ＧＦＡＰ） 基因表达对在体胶质瘢痕形成的影响。方法　构建反义ＧＦＡＰ逆转录病毒表达载体，经过病毒包装后，将获得的病毒上清液浓缩并通过微量注射的方法，引入大鼠脑穿刺损伤灶内，采用免疫组化及形态学观察的方法，研究重组反义ＧＦＡＰ逆转录病毒对脑损伤后胶质瘢痕形成的影响。　结果　重组反义ＧＦＡＰ逆转录病毒使脑穿刺损伤灶周围ＧＦＡＰ阳性细胞数量明显减少，反应性胶质化的典型特征消失，胶质瘢痕形成明显减少。　结论　重组反义ＧＦＡＰ逆转录病毒能在一定程度上减少胶质瘢痕形成，为利用基因工程技术延缓或抑制胶质瘢痕形成提供了新的实验依据。 Objective To study the effect of inhibiting the expression of glial fibrillary acidic protein (GFAP) on the formation of glial scar. Methods The antisense GFAP retrovirus expression vector was constructed. After viral packaging, the obtained virus supernatant was concentrated and injected into the lesion of brain puncture by microinjection. Immunohistochemistry and morphological observation To study the effect of recombinant antisense GFAP retrovirus on glial scar formation after brain injury. Results The recombinant retroviral GFAP retrovirus significantly reduced the number of GFAP positive cells around the brain puncture lesion. The typical features of reactive glia disappeared and glial scar formation was significantly reduced. Conclusion Recombinant antisense GFAP retrovirus can reduce the formation of glial scar to a certain extent, and provide a new experimental basis for using gene engineering technology to delay or inhibit glial scar formation.