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猪气喘病是由猪肺炎支原体引起的一种慢性、接触性呼吸道传染病,其流行范围广、难治愈,给养猪业造成了巨大的经济损失。本试验在前期RNA测序结果的基础上,首先选出了差异表达基因干扰素γ受体1(INFGR1),利用PCR扩增和PCR产物直接测序筛查了INFGR1基因外显子区域的SNP位点,进一步在苏淮猪群体开展了基因型分型检测,同时利用实时荧光定量PCR技术检测了苏淮猪群体的猪肺炎支原体感染水平,最后统计分析了INFGR1基因SNP位点不同基因型与苏淮猪群体猪肺炎支原体感染水平的相关。结果发现在苏淮猪IFNGR1基因外显子5上检测到一个SNP(A12557G),苏淮猪群体A等位基因频率为0.555,G等位基因频率为0.445;不同基因型猪肺炎支原体感染量差异极显著(P<0.01),GG基因型感染水平显著高于AA基因型,初步推测IFNGR1基因SNP(A12557G)可作为影响苏淮猪气喘病抗性选育潜在的分子遗传标记。
Swine asthma is caused by Mycoplasma hyopneumoniae is a chronic, contagious respiratory disease, its wide range of epidemic, difficult to cure, to the pig industry caused tremendous economic losses. In this study, based on the results of previous RNA sequencing, we first selected the differentially expressed interferon gamma receptor 1 (INFGR1), using PCR amplification and direct sequencing of PCR products to screen the SNP sites in the exon of the INFGR1 gene , Further genotypes were detected in Suhuai pig population, and the level of Mycoplasma hyopneumoniae infection in Suhuai pig population was detected by real-time fluorescence quantitative polymerase chain reaction (PCR). Finally, the genotypes of SNPs of INFGR1 gene were compared with those of Su-Huai Correlation of Mycoplasma hyopneumoniae infection in swine. The results showed that a SNP (A12557G) was detected on exon 5 of IFNGR1 gene of Suhuai pig. The frequency of allele A was 0.555 and the frequency of G allele was 0.445 in Suhuai pig population. The differences in the infection rates of Mycoplasma hyopneumoniae between different genotypes (P <0.01). The GG genotype infection level was significantly higher than that of the AA genotype. It was preliminarily presumed that IFN-γ gene SNP (A12557G) could be used as a potential molecular genetic marker to influence the asthma resistance of Suhuai pig.