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目的:探讨曲普瑞林对子宫肌瘤患者肿瘤组织中MMP-13和TIMP-3表达影响。方法:选取我院收治的子宫肌瘤患者40例,随机分为两组,每组各20例。实验组予曲普瑞林后,行子宫肌瘤切除术,对照组直接行子宫肌瘤切除术,观察比较超声测量子宫和子宫肌瘤的体积,实时定量PCR法与免疫印迹法检测子宫肌层和肌瘤组织中MMP-13和TIMP-3的表达。结果:1与曲普瑞林治疗前以及对照组相比较,曲普瑞林治疗后临床症状显著改善,子宫和肌瘤体积均明显缩小,差异有统计学意义(P<0.05)。2与对照组和治疗前相比较,血清雌二醇水平显著降低,差异有统计学意义(P<0.05)。3与对照组相比较,肌层和肌瘤组织中MMP-13m RNA水平降低,肌层和肌瘤组织中TIMP-3m RNA水平增加,差异有统计学意义(P<0.05)。结论:曲普瑞林能够通过降低血清雌二醇水平,降低肌层和肌瘤组织中MMP-3的表达,以及增加肌层和肌瘤组织中TIMP-13的表达,来缩小子宫和子宫肌瘤的体积,从而缓解患者的症状。
Objective: To investigate the effect of triptorelin on the expression of MMP-13 and TIMP-3 in the tumor tissue of patients with uterine fibroids. Methods: Forty patients with uterine leiomyoma admitted to our hospital were randomly divided into two groups with 20 cases in each group. The experimental group was treated with triptorelin, myomectomy, the control group directly underwent myomectomy, the volume of uterine and uterine fibroids were observed and compared by ultrasound quantitative real-time PCR and immunoblotting to detect the myometrium And fibroid tissue MMP-13 and TIMP-3 expression. Results: 1 Compared with triptorelin before treatment and control group, triptorelin treatment significantly improved clinical symptoms, uterine and fibroid volume were significantly reduced, the difference was statistically significant (P <0.05). 2 Compared with the control group and before treatment, serum estradiol level was significantly lower, the difference was statistically significant (P <0.05). 3 Compared with the control group, the level of MMP-13mRNA in myometrium and myoma decreased, and the level of TIMP-3mRNA in myometrium and myoma increased (P <0.05). Conclusion: Triptorelin can reduce uterine and uterine muscles by decreasing serum estradiol level, decreasing the expression of MMP-3 in myometrium and fibroid tissue, and increasing the expression of TIMP-13 in myometrium and fibroid tissue The size of the tumor, thus alleviating the symptoms of the patient.