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目的:探讨牛磺酸脱氧胆酸(TDCAO)对HepG2细胞凋亡和坏死的影响。方法:应用台盼蓝拒染试验判断细胞坏死,应用HE染色、Hoechst 33 258染色、电镜和DNA电泳对细胞凋亡定性;应用流式细胞仪对细胞凋亡定量。结果:TDCA 200 μmol/L孵育15 h可抑制细胞生长及增殖;400 μmol/L孵育 12 h可诱导显著 HepG2细胞凋亡,凋亡率为50.35±2.20％;600μmol/L孵育6 h即显著抑制细胞生长和增殖,随孵育时间延长脱落坏死细胞明显增多;800μmol/L细胞不能增殖,孵育6 h即可引起细胞坏死。结论:TDCA与HepC2细胞孵育时,即可引起细胞坏死,又可诱导细胞凋亡,取决于浓度及孵育时间的长短;在低浓度,TDCA对细胞的毒性主要表现在为抑制细胞生长增殖和诱导细胞凋亡,在高浓度则以引起细胞坏死为主。
Objective: To investigate the effect of taurodeoxycholic acid (TDCAO) on apoptosis and necrosis in HepG2 cells. Methods: Cell apoptosis was determined by trypan blue exclusion test. HE staining, Hoechst 33 258 staining, electron microscopy and DNA electrophoresis were used to characterize cell apoptosis. Flow cytometry was used to quantify apoptosis. Results: After incubated with 200 μmol / L TDCA for 15 h, the cell growth and proliferation were inhibited. After incubation with 400 μmol / L for 12 h, significant apoptosis of HepG2 cells was induced with a apoptosis rate of 50.35 ± 2.20% Cell growth and proliferation, with the extension of incubation time off necrotic cells increased significantly; 800μmol / L cells can not proliferate, 6 h incubation can cause cell necrosis. Conclusion: TDCA and HepC2 cells can cause cell necrosis and apoptosis when incubated with HepC2 cells, depending on concentration and incubation time. At low concentrations, the toxicity of TDCA to cells is mainly in the inhibition of cell proliferation and induction Apoptosis, at high concentrations to cause cell-based necrosis.