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目的 探讨胃粘膜癌变过程中细胞增殖活性的变化以及PCNA、P5 3基因在胃癌发病过程中的意义。方法 采用免疫组化 (Immunohistochemistry)方法对临床 95例胃手术切除标本PCNA、P5 3基因的表达情况进行检测分析。结果 胃癌变过程中均可见PCNA的阳性表达 ,随病变程度的加重 ,PCNA增殖指数亦逐渐上升 ,各组间差异显著 (P <0 .0 5 ) ,但重度异型增生与胃癌组织相比较 ,PCNA指数变化不大 (P >0 .0 5 )。仅在重度异型增生组织中发现P5 3蛋白表达 ,胃癌P5 3蛋白的表达阳性率明显增高 ,与重度异型增生组织相比较 ,具有显著性差异 (P <0 .0 5 )。结论 在胃癌变过程中 ,细胞增殖活性逐渐增高。对临床标本进行PCNA检测 ,可较方便准确地判断组织的增殖状况。P5 3蛋白表达出现较晚 ,其可能表明P细胞已有明显的恶性转化
Objective To investigate the changes of cell proliferation activity in gastric carcinogenesis and the significance of PCNA and P53 gene in the pathogenesis of gastric cancer. Methods Immunohistochemistry was used to detect the expression of PCNA and P53 gene in 95 cases of clinical surgical resection. Results The positive expression of PCNA was observed in the course of gastric cancer. As the severity of the lesions increased, the PCNA proliferation index also increased gradually. The difference between the groups was significant (P < 0.05), but severe dysplasia was compared with gastric cancer. PCNA The index did not change much (P > 0.05). The expression of P53 protein was only found in severe dysplastic tissues. The positive rate of P53 protein expression in gastric cancer was significantly higher than that in severe dysplasia tissues (P < 0.05). Conclusion In the process of gastric cancer, cell proliferation activity gradually increased. PCNA detection of clinical specimens can easily and accurately determine the proliferation of tissues. P5 3 protein expression appears later, which may indicate that the P cells have obvious malignant transformation