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目的 探讨“彗星”分析法在检测人癌体外培养细胞放射敏感性方面的应用价值。方法应用“彗星”法对人鼻咽高分化鳞状细胞癌CNE 1和肺腺癌 973细胞进行检测 ,以尾力矩作为定量评价指标 ,检测细胞照射后的DNA初始损伤和损伤后的修复能力 ;同时 ,用经典的克隆形成方法测算CNE 1和 973细胞的D0 值和Dq值 ,并对两种实验方法所得结果进行比较。结果 (1)“彗星”法测定显示 ,CNE 1细胞的初始DNA损伤大于 973细胞 ,差异有显著性 (P <0 .0 1) ,提示CNE 1细胞的放射敏感性高于 973细胞 ;细胞存活曲线检测显示 ,CNE 1细胞的D0 值为 1.6 31,973细胞的D0 值为 1.82 2 ,提示CNE 1细胞的放射敏感性高于 973细胞。两种方法检测结果所反映细胞放射敏感性的趋势一致。(2 )“彗星”分析法检测显示 ,973细胞DNA损伤的半修复时间为 33min ,CNE 1细胞的半修复时间为 4 1min ,提示 973细胞对DNA损伤的修复能力强于CNE 1细胞 ;细胞存活曲线显示 ,973细胞的Dq值为2 .15 2 ,CNE 1细胞的Dq值为 0 .6 2 6 ,提示 973细胞的亚致死损伤修复能力大于CNE 1细胞。两种方法测定结果所反映的损伤修复能力的差异也一致。结论 “彗星”分析法检测CNE 1和 973细胞的DNA初始损伤及修复所反映的放射敏感性 ,与经典细胞存活曲线法所测的放射敏?
Objective To investigate the value of “comet assay” in detecting the radiosensitivity of cultured human cancer cells in vitro. Methods “Comet” method was used to detect human nasopharyngeal adenocarcinoma well-differentiated squamous cell carcinoma CNE 1 and lung adenocarcinoma 973 cells. Tail torque was used as a quantitative evaluation index to detect initial DNA damage and repair ability after injury. At the same time, D0 and Dq values of CNE1 and 973 cells were measured by classical clonogenic methods, and the results obtained by the two experimental methods were compared. Results (1) The comet assay showed that the initial DNA damage of CNE 1 cells was greater than that of 973 cells (P <0.01), which indicated that the radiosensitivity of CNE 1 cells was higher than that of 973 cells. Curvilinear analysis showed that the D0 value of CNE 1 cells was 1.631 and the D0 value of 973 cells was 1.82 2, suggesting that the radiosensitivity of CNE 1 cells was higher than that of 973 cells. The results of the two methods reflect the trend of cell radiosensitivity consistent. (2) “Comet” assay showed that the half repair time of DNA damage of 973 cells was 33 min and the half repair time of CNE 1 cells was 41 min, which indicated that 973 cells were more capable of repairing DNA damage than CNE 1 cells. The curve shows that the Dq value of 973 cells is 2.152, and the Dq value of CNE 1 cells is 0.666, suggesting that 973 cells have a greater ability of repairing sublethal damage than CNE 1 cells. The difference between the damage repair capabilities reflected by the results of the two methods is also consistent. Conclusion The “comet assay” was used to detect the initial DNA damage in CNE 1 and 973 cells and the radiosensitivity of the repaired cells. The radiosensitivity measured by the classical cell survival curve method