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目的探讨乙肝病毒（ＨＢＶ）与肝癌的相关性．方法取用乙肝疫苗注射３次前后外周血淋巴细胞（ＰＢＬ）；ＨＢＶＤＮＡ转染的ＨｅｐＧ２２２１５株培养上清（ＨＢｓＡｇ，ＨＢｅＡｇ阳性）感染健康人ＰＢＬ后进行ＴＣＲＶβ基因１－２０亚家族表达水平的分析．结果乙肝疫苗注射后及ＨｅｐＧ２２２１５株培养上清感染健康人ＰＢＬ后ＴＣＲＶβ６，１４；Ｖβ６，１５特异性扩增而表达水平显著增高．结论Ｖβ６可能为识别ＨＢＶ抗原或引发免疫应答的基因片段，但Ｖβ１４，１５各自在限制和杀伤功能方面起着重要的作用，这从分子水平上又证明了ＨＢＶ与肝癌的关系
Objective To explore the relationship between hepatitis B virus (HBV) and liver cancer. Methods The expression levels of TCR Vβ gene 1-20 subfamily were detected in peripheral blood lymphocytes (PBL) before and after three injections of hepatitis B vaccine, and HBV DNA transfected HepG22215 culture supernatant (HBsAg, HBeAg positive) infected healthy human PBL. Results The expression levels of TCRVβ6,14 and Vβ6,15 were specifically amplified and the expression level was significantly increased after the injection of HepG22215 and the infection of HepG22215 strain. Conclusion Vβ6 may be a gene fragment that recognizes HBV antigen or elicited an immune response, but Vβ14, 15 each plays an important role in limiting and killing functions. This proves the relationship between HBV and liver cancer at the molecular level.