论文部分内容阅读
目的 :了解口腔颌面部肿瘤组织中 型纤溶酶原激活剂抑制剂 (palsminogen activator inhibitor- 1,PA - 1)蛋白表达情况 ,分析其与各病理参数间的关系。方法 :采用发色底物法、EL ISA夹心法测定 30例口腔颌面部恶性肿瘤组织、相应瘤旁组织及 10例良性肿瘤组织的 PA - 1活性及含量。结果 :恶性肿瘤组织 PA - 1活性及含量明显高于良性肿瘤及瘤旁组织 (P<0 .0 1) ;PA - 1活性及含量与临床分期相关(P<0 .0 1和 P<0 .0 1) ,与颈淋巴结转移明显相关 (P<0 .0 1)。结论 :PA - 1在口腔颌面部恶性肿瘤中有高表达 ,且与肿瘤的侵袭和转移有关 ,在蛋白水平上检测 PA - 1对判断有无转移、指导手术、评估预后有帮助作用。
OBJECTIVE: To investigate the expression of palsminogen activator inhibitor - 1 (PA - 1) in oral and maxillofacial tumors and to analyze its relationship with pathological parameters. Methods: The activity and content of PA - 1 in 30 cases of oral and maxillofacial malignant tumor tissues, corresponding para - tumor tissues and 10 cases of benign tumor tissues were determined by ELISA sandwich method using chromogenic substrate method. Results: The activity and content of PA - 1 in malignant tumor tissue were significantly higher than those in benign tumor and adjacent tumor tissue (P <0.01). The activity and content of PA - 1 were correlated with clinical stage (P <0.01 and P <0.01) .0 1), which was significantly associated with cervical lymph node metastasis (P <0.01). CONCLUSION: PA - 1 is overexpressed in oral and maxillofacial malignant tumors and is related to tumor invasion and metastasis. Detecting PA - 1 at the protein level may be useful in judging the presence or absence of metastasis, guiding the operation and assessing prognosis.