论文部分内容阅读
目的探索酶学生物标志物精氨酸酶玉(Arginase玉,Arg玉)、琢-谷胱甘肽S转移酶(琢-glutathione-S-transferase,琢-GST)、谷胱苷肽脱氢酶(Glutamic dehydrogenase,GLDH)和嘌呤核苷磷酸酶(purine nucleosidephosphorlyase,PNP)作为吴茱萸致肝损伤早期敏感指标的可能性。方法取KM小鼠,随机分为正常组和吴茱萸组,吴茱萸组连续28 d灌胃给予吴茱萸30 g·kg-1·d-1,正常组灌服等体积蒸馏水;取Wistar大鼠,随机分为正常组和吴茱萸组,吴茱萸组连续28 d灌胃给予吴茱萸20 g·kg-1·d-1,正常组灌服等体积蒸馏水,各组均于给药后不同时间点取血及肝组织。全自动生化分析仪检测动物血清生化指标:丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)和总胆红素(TB)含量,ELISA法检测血清中Arg玉、琢-GST、GLDH和PNP的含量。HE染色,光镜观察肝组织病理形态学变化。结果小鼠实验中,与同时间点对照组相比,吴茱萸组ALT于给药21 d和28 d显著性升高(P约0.05或P约0.01),肝组织形态学检查仅见个别小鼠的肝细胞坏死。吴茱萸组血清生物标志物Arg玉、琢-GST和PNP于7 d起明显升高,GLDH于14 d起明显升高(P约0.05或P约0.01),均持续至28 d。大鼠实验中,与同时间点正常组比较,吴茱萸组常规生化指标均无明显变化,肝组织形态学检查仅极少数动物见肝细胞坏死,吴茱萸组血清生物标志物Arg玉、GLDH和PNP于7 d起明显升高,琢-GST于14 d起明显升高(P约0.05,P约0.01),均持续至28 d。结论血清Arg玉、琢-GST、GLDH和PNP可作为吴茱萸致肝损伤的早期敏感酶学生物标志物。
Objective To explore the role of enzymatic biomarker arginase jade (Arginase jade, Arg jade), glutathione S-transferase (Zhuo-GST), glutathione dehydrogenase Glutamic dehydrogenase (GLDH) and purine nucleosidephosphorlyase (PNP) as early warning indicators of liver injury induced by Evodia. Methods KM mice were randomly divided into normal group and Evodia rutaecarp group. Evodia rutaefolium was given intragastrically to Evodia 30 g · kg-1 · d-1 for 28 days. The rats in normal group were given distilled water in equal volume. Wistar rats were randomly divided into three groups In the normal group and evodiamine group, evodia officinalis group was given intragastric administration of Evodia rutaecarpa 20 g · kg-1 · d-1 for 28 days. The normal group was fed with equal volume of distilled water. The rats in each group were given blood and liver tissue at different time points . The automatic biochemical analyzer was used to detect the serum biochemical indicators of animals: alanine aminotransferase (ALT), aspartate aminotransferase (AST) and total bilirubin (TB) content, ELISA method to detect serum Arg jade, -GST, GLDH and PNP content. HE staining, light microscopy liver histopathological changes. Results Compared with the control group at the same time, the ALT of Evodia was significantly increased at 21 d and 28 d after administration (P was about 0.05 or P was about 0.01). Liver morphology was only found in the Hepatocyte necrosis. The serum biomarkers Arg Jade, Zhuo-GST and PNP of Evodia rutaecarpa significantly increased from day 7, and the GLDH increased significantly from day 14 (P about 0.05 or P about 0.01), both lasting for 28 days. Compared with the normal group, the conventional biochemical indexes of Evodia rutaecarpa group did not change significantly. Only a few animals in liver tissue morphology showed necrosis of hepatocytes. The serum biomarkers Arg jade, GLDH and PNP in Evodia Significantly increased on day 7 and significantly increased at 14 days (P = 0.05, P = 0.01), all lasting up to 28 days. Conclusion Serum Arg Jade, Zhuo-GST, GLDH and PNP can be used as early sensitive enzyme biomarkers of liver injury induced by Evodia.