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采用山西栽培的５个地方名蒜茎尖离体培养，获得了无病毒蒜种。结果表明，茎尖大小为０．２～０．５ｍｍ，不定芽增殖培养基以Ｂ５或ＭＳ．附加１～２ｍｇ／Ｌ细胞分裂素和０．０１～０．１ｍｇ／ＬＮＡＡ，效果最佳。根系诱导在原培养基中加入０．２ｍｇ／ＬＮＡＡ，出根率可达９０％以上。成苗后于１２月份移栽到节能日光温室，成活率达９０％以上。经对脱毒苗形态观察和染色体检测，没有发生异常变化，植株生长健壮，遗传性稳定，产量高。
Five local cultivated garlic root tips were cultured in vitro to obtain virus-free garlic. The results showed that shoot tip size was 0.2 ~ 0.5mm, adventitious bud proliferation medium was B5 or MS. Addition of 1 ~ 2mg / L cytokinin and 0.01 ~ 0.1mg / LNAA, the best. Root induction in the original medium by adding 0.2mg / LNAA, root out rate of up to 90%. Transplanted into energy-saving solar greenhouse in December after the seedling, the survival rate of more than 90%. After the morphological observation of virus-free seedlings and chromosomal detection, no abnormal changes occurred, the plant grew robust, hereditary stability, high yield.