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目的研究泽兰有效部位L .F0 4对血小板聚集和血栓形成的影响 ,以探讨其活血化瘀作用机理。方法用高分子右旋糖酐静脉推注造成大鼠血瘀证动物模型 ,观察泽兰L .F0 4对ADP诱导的大鼠体内血小板聚集以及体内动静脉旁路血栓、体外旋转环内血栓形成的影响。结果L .F0 4 0 .40 8g/kg、0 .2 0 4g/kg对模型组大鼠ADP诱导的体内血小板最大聚集率明显增加皆有显著的抑制 ,且呈剂量依赖关系 ;与对照组相比 ,血瘀模型大鼠体外血栓重量明显增加 ,长度仅有增加趋势 ,L .F0 4 0 .40 8g/kg、0 .2 0 4g/kg皆有抗血栓形成作用 ,L .F0 4 0 .40 8g/kg对血栓干重、湿重的减轻尤为明显 ;L .F0 4 0 .40 8g/kg、0 .2 0 4g/kg对实验性动静脉旁路血栓形成均有明显的抑制作用 ,抑制率分别为 2 7.41 %、2 7.1 4%。结论泽兰L .F0 4可显著抑制血小板聚集及体内、外血栓形成
Objective To study the effect of L. F0 4 on the platelet aggregation and thrombosis in Eupatorium zeae to explore its mechanism of promoting blood circulation and removing blood stasis. Methods Animal model of blood stasis syndrome was induced by intramuscular injection of high molecular dextran. The effect of Zeelandia L. F0 4 on platelet aggregation in rats induced by ADP, arteriovenous shunt thrombosis in vivo and thrombosis in vitro were studied. Results L.F0 4 0 .40 8g/kg, 0. 204g/kg significantly inhibited ADP-induced platelet maximal aggregation in the model group in a dose-dependent manner, and compared with the control group. In comparison, the thrombus weight in blood stasis model rats increased significantly, and the length increased only. L.F0 40.40 8g/kg, 0.24g/kg had antithrombotic effects, L.F0 4 0 . The reduction of thrombus dry weight and wet weight was especially obvious at 40 8 g/kg; L.F04.40 8 g/kg, 0.24 g/kg had obvious inhibitory effects on experimental arteriovenous shunt thrombosis. The inhibition rates were 27.14% and 2 7.14%, respectively. Conclusion Zeeland L.F0 4 can significantly inhibit platelet aggregation and in vitro and in vivo thrombosis.