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背景:中缝大核是延脑腹侧中缝处的一个重要核团,在针刺镇痛、心血管活动调节、呼吸运动调节等方面具有重要的作用,但目前有关其在消化功能调节中的作用报道很少。目的:探讨中缝大核对家兔胃运动及胃电活动的调节作用。设计:以实验动物为研究对象的观察对比实验。单位:一所中医药大学的机能实验室。对象:本实验于2002-09/2003-04在黑龙江中医药大学机能实验室(省重点实验室)完成。选择健康新西兰白兔70只,体质量2.0～2.5kg,雌雄不拘,由黑龙江中医药大学动物中心(Ⅰ级)提供,实验中随机分为7组,即生理盐水组、5-羟色胺组、赛庚啶组、P物质组、P物质拮抗剂组、吗啡组、去甲肾上腺素组。方法:通过中缝大核插管,分别向中缝大核内注射微量的5-羟色胺、赛庚啶、P物质、P物质拮抗剂、吗啡和去甲肾上腺素,并利用霍尔效应原理同步描记家兔胃运动、胃电慢波的波幅与频率变化。主要观察指标:中缝大核区微量注射5-羟色胺、赛庚啶、P物质、P物质拮抗剂、吗啡及去甲肾上腺素后胃运动、胃电的变化。结果:中缝大核微量注入5-羟色胺、P物质可抑制家兔的胃运动和胃电慢波(P<0.05,P<0.01)。中缝大核微量注入赛庚啶、P物质拮抗剂后可使家兔的胃运动和胃电慢波增强(P<0.05,P<0.01)。中缝大核微量注入吗啡、去甲肾上腺素后对家兔
Background: The dorsal raphe nucleus is an important nucleus in the ventral midline of the ventral tegmental artery and plays an important role in acupuncture analgesia, cardiovascular activity regulation and respiratory regulation. However, its role in the regulation of digestive function Few reports. Objective: To investigate the regulation effect of the nucleus raphe magnus on gastric motility and gastric electrical activity in rabbits. Design: Observations and experiments comparing experimental animals. Unit: a functional laboratory of Chinese medicine University. PARTICIPANTS: The experiment was performed in Functional Laboratory of Heilongjiang University of Chinese Medicine (Provincial Key Laboratory) from September 2002 to April 2003. A total of 70 healthy New Zealand white rabbits were selected and their body weight was 2.0-2.5 kg. They were provided by Animals Center of Heilongjiang University of Chinese Medicine (grade Ⅰ). The rabbits were randomly divided into 7 groups: normal saline group, serotonin group, Heptyl group, substance P group, substance P antagonist group, morphine group and norepinephrine group. METHODS: A small amount of 5-hydroxytryptamine, cyproheptadine, substance P, substance P antagonist, morphine and norepinephrine were injected into the nucleus raphe nuclei through the large nucleus cannula. Rabbit gastric motility, gastric electrical slow wave amplitude and frequency changes. MAIN OUTCOME MEASURES: Changes of gastric motility and gastric electrical activity after microinjection of serotonin, cyproheptadine, substance P, substance P antagonist, morphine and norepinephrine into the nucleus raphe magnus. Results: Serotonin and substance P could inhibit gastric motility and gastric electrical slow wave (P <0.05, P <0.01). Microinjection of cyproheptadine and substance P antagonist into the nucleus raphe increased gastric motility and gastric electrical slow wave (P <0.05, P <0.01). In the middle of the nucleus micro-injection of morphine, norepinephrine after the rabbit