论文部分内容阅读
目的建立同时测定新型浓苯甲酸水杨酸软膏中苯甲酸和水杨酸浓度的RP-HPLC法。方法采用Kromasil-C_(18)(150 mm×4.6 mm,5μm)色谱柱;流动相为V(甲醇):V(0.02 mol·L~(-1),KH_2PO_4溶液,磷酸调pH=3.1)=50:50;检测波长280nm;流速1.0 mL·min~(-1);柱温20℃。结果苯甲酸、水杨酸进样质量浓度分别为96～144 mg·L~(-1)(r=0.999 5)、48～72 mg·L~(-1)(r=0.999 7)时具良好线性关系,加样回收率分别为99.6%～100.6%、99.4%～100.2%,批间、批内RSD为0.3%～0.7%。结论本方法简便、精确、重现性好,适用于测定新型浓苯甲酸水杨酸软膏中苯甲酸和水杨酸浓度。
OBJECTIVE To establish a RP-HPLC method for simultaneous determination of benzoic acid and salicylic acid in salicylic acid ointment. Methods Kromasil-C 18 column (150 mm × 4.6 mm, 5 μm) was used. The mobile phase consisted of V (methanol): V (0.02 mol·L -1, KH 2 PO 4) 50:50; detection wavelength 280nm; flow rate 1.0 mL · min -1; column temperature 20 ℃. Results The injection concentrations of benzoic acid and salicylic acid were 96 ~ 144 mg · L -1 (r = 0.999 5), 48 ~ 72 mg · L -1 (r = 0.999 7) Good linear relationship, sample recovery rates were 99.6% ~ 100.6%, 99.4% ~ 100.2%, intra-batch, intra-batch RSD 0.3% ~ 0.7%. Conclusion The method is simple, accurate and reproducible. It is suitable for the determination of benzoic acid and salicylic acid in salicylic acid ointment.