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目的 通过免疫组化的方法 ,研究APP1 7肽对链佐星 (STZ)诱发的糖尿病大鼠海马神经元Akt PKB ,CREB ,GSK 3β以及Bax ,Bcl 2等蛋白表达的影响。方法 用STZ腹腔注射诱发糖尿病模型 ,并皮下注射APP1 7肽对糖尿病组进行治疗。 1 0周后行通道水迷宫测定 ,取脑组织进行Akt PKB ,GSK 3β以及Bax ,CREB ,Bcl 2的免疫组化染色。结果 糖尿病组海马内Akt PKB ,CREB的阳性反应神经元数目减少 ,染色淡。GSK 3β,Bax阳性神经元较对照组明显增多 (P <0 .0 1 )。Bcl 2阳性神经元数目 3组间相比无明显差异。糖尿病APP1 7肽治疗组与对照组相近。结论 糖尿病脑病大鼠海马存在胰岛素信号转导途径相关和凋亡级联反应相关蛋白的表达异常 ;APP1 7肽能改善这些蛋白表达 ,使之接近正常
Objective To investigate the effect of APP1 7 peptide on the expression of Akt PKB, CREB, GSK 3β, Bax and Bcl 2 in hippocampal neurons induced by streptozotocin (STZ) in rats by immunohistochemistry. Methods The diabetes model was induced by intraperitoneal injection of STZ and the APP1 7 peptide was administered subcutaneously to the diabetic group. The water maze was measured in the passageway after 10 weeks. The brain tissues were taken for immunohistochemical staining of Akt PKB, GSK 3β, Bax, CREB and Bcl 2. Results The number of positive neurons of Akt PKB and CREB in the hippocampus of diabetic group decreased and the staining was light. GSK 3β, Bax-positive neurons were significantly increased compared with the control group (P <0.01). Bcl 2-positive neurons no significant difference between the three groups. Diabetes APP1 7 peptide treatment group and control group similar. Conclusions The hippocampus of diabetic encephalopathy rats have the abnormal expression of insulin signal transduction pathway and related proteins of apoptotic cascade. APP1 7 peptide can improve the expression of these proteins and make them close to normal