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目的观察乙烷硒啉对大鼠配子成熟、交配行为、生育力和早期胚胎发育方面的毒性影响。方法乙烷硒啉设0,250,500和1000 mg/kg组。雄性大鼠连续灌胃给药8周,雌性大鼠连续灌胃给药2周,进行组内同笼交配,共交配2个性周期。交配期间继续给药至妊娠第14 d处死,解剖检查卵巢、子宫和胚胎等生殖器官。在确定雌鼠受精后处死同笼的雄鼠,解剖检查雄性睾丸和附睾等生殖器官。结果给药期间各剂量组大鼠体重增长缓慢(平均最大差值达到-70 g);雄性大鼠生殖器官发育良好,性功能正常,精子数量多、成熟,畸形率低。妊娠大鼠各剂量组的活胎数明显减少(窝平均最大差值达到-6.8个)、吸收胎数增多(窝平均最大差值达到+5.9个)和受精卵着床后丢失率增加(>54%);但交配率、妊娠率、黄体数、着床数、死胎数、胎仔体重和胎仔体表畸形数等与对照组基本一致。结论乙烷硒啉可影响雄性成年大鼠体重增长,引起妊娠大鼠早期胚胎发育障碍和受精卵着床后丢失;但对大鼠其他生殖功能无明显损害作用。
Objective To observe the effects of ethaselen on the toxicity of gametogenesis, mating behavior, fertility and early embryo development in rats. Methods Ethaselen was divided into 0, 250, 500 and 1000 mg / kg groups. The male rats were given gavage for 8 weeks. The female rats were given gavage for 2 weeks. The mice were mated with the same cage for 2 cycles. During the mating period, the drug will be administered to the 14th day of pregnancy, and the reproductive organs such as ovary, uterus and embryo are dissected. After determining female fertilization male rats were sacrificed with the same cage, anatomical examination of male testes and epididymis and other reproductive organs. Results The body weight of rats in each dose group increased slowly (the average maximum difference reached -70 g) during the administration. The reproductive organs of male rats developed well, their sexual function was normal, the number of sperm was large, their maturation was low, and the rate of deformity was low. The number of live fetuses in each dose group of pregnant rats decreased significantly (the average maximum difference between the litters was -6.8), the number of fetuses increased (the maximum difference between litters reached +5.9) and the rate of lost after fertilization (> 54%). However, the mating rate, pregnancy rate, luteal number, implantation number, stillbirth, fetal body weight and fetal body malformation were basically the same as the control group. Conclusion Ethaselen can affect the weight gain of male adult rats, cause the embryo developmental disorders of pregnant rats and the loss of fertilized eggs after implantation, but have no significant effect on other reproductive functions in rats.