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采用T4 BSA联结物免疫BALB/c小鼠 ,通过杂交瘤技术 ,获得 4株稳定分泌高特异性抗T4单克隆抗体的杂交瘤细胞系 ,并制取到含高效价单克隆抗体的腹水。放免分析结果显示 ,所得的 4株单抗对T4均表现高亲和性和高特异性 :Ka>10 8L/Mol,与T2交叉反应未测出 ,与T3交叉反应 <0 4 0 % ,与rT3交叉反应为 <0 2 2 %。采用T4 10D11株单抗替代多抗应用于放免药盒 (RIA )与商品药盒进行比较 ,同时测定 96例临床血样 ,结果表明 ,两者之间有极显著的正相关 (Y =1 12X 3 15 ,r=0 985 0 ) ,为这些单抗的应用提供了基础
BALB / c mice were immunized with T4 BSA conjugate. Four hybridoma cell lines stably secreting high specific anti-T4 monoclonal antibody were obtained by hybridoma technique and ascitic fluid containing high titer monoclonal antibody was obtained. The results of radioimmunoassay showed that the four McAbs obtained showed high affinity and high specificity to T4 with Ka> 10 8L / Mol, no cross-reaction with T2, no cross-reaction with T3, and The rT3 cross-reactivity was <0 2 2%. The monoclonal antibody of T4 10D11 was used in place of polyclonal antibody to compare RIA with commercial kits, and 96 clinical blood samples were also determined. The results showed that there was a significant positive correlation between them (Y = 1 12X 3 15, r = 0 985 0) provided the basis for the use of these mAbs