论文部分内容阅读
Human marrow stromal cells (hMSCs) are multipotential stem cells,capable of differentiating into bone,cartilage,fat and muscle.Several recent reports demonstrated that hMSCs have been also differentiated into neural cells.However,only a few reported inducers are applicable for clinical use.This work is to explore the effects of sodiumferulate (SF) on differentiation of hMSCs into neural cells in vitro.We found that hMSCs could be induced to thecells with typical neural morphology when cultured with SF.The cells express neural proteins,such as nestin,neuron-specific enolase (NSE) and glial fibrillary acidic protein (GFAP).About 30% of the hMSC-derived cellsexpressed nestin when cultured with SF for 3 h,but no expression was detected after 24 h.The percentages ofpositive cells for NSE or GFAP were about 67% and 39% separately at 6 h,and reached the plateau phage aftertreatment with SF for 3 days.The data suggest that SF can induce hMSCs to differentiate into neural-like cells invitro.Cellular & Molecular Immunology.2005;2(3):225-229.
Human marrow stromal cells (hMSCs) are multipotential stem cells,capable of differentiating into bone,cartilage,fat and muscle.Several recent reports showed that hMSCs have been also differentiated into neural cells.However, only a few reported inducers are applicable for clinical use This work is to explore the effects of sodiumferulate (SF) on differentiation of hMSCs into neural cells in vitro.We found that hMSCs could be induced to the cells with typical neural morphology when cultured with SF. The cells express neural proteins,such as nestin ,neuron-specific enolase (NSE) and glial fibrillary acidic protein (GFAP).About 30% of the hMSC-derived cellsexpressed nestin when cultured with SF for 3 h, but no expression was detected after 24 h.The percentages of positive cells for NSE Or GFAP were about 67% and 39% separately at 6 h, and reached the plateau phage aftertreatment with SF for 3 days.The data suggest that SF can induce hMSCs to differentiation into neural-like cells invitro.Cel Lular & Molecular Immunology. 2005;2(3):225-229.