论文部分内容阅读
17β-羟甾脱氢酶（17β-HSD）催化雄甾烯二酮转化成睾酮，已知Ⅰ型和Ⅲ型17β－HSD为催化还原反应的同功酶，本研究测定了小鼠胚胎期和出生后这二种还原型同功酶的mRNA水平。在胚胎期和刚出生的睾丸，Ⅰ型和Ⅲ型同功酶的mRNA均为高表达，出生后第5天为峰值，而后，二者的表达明显降低并持续到出生第30天，第30天起，Ⅲ型同功酶的表达明显增高。结果提示：在缺乏Ⅲ型同功酶而致的男性假二性畸型中，胚胎期Ⅰ型17β－HSD的表达可能与Wolffian管的分化有关。
17β-hydroxysteroid dehydrogenase (17β-HSD) catalyzes the conversion of androstenedione to testosterone. Type I and type III 17β-HSD are known as isoenzymes for catalytic reduction. In this study, The mRNA levels of these two reduced isozymes after birth. In embryonic and newborn testis, mRNAs of type I and type III isozymes were all highly expressed, and peaked on the fifth day after birth, after which the expression of both was significantly reduced and continued until the 30th day of birth, 30 From the beginning, the expression of type Ⅲ isozyme was significantly increased. The results suggest that in the absence of type Ⅲ isozymes in males, the expression of type I 17β-HSD may be related to Wolffian tube differentiation.