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棉花曲叶病毒 (CLCuV)是一种单链DNA病毒 ,属于双生病毒亚组Ⅲ。检测了双生病毒双向启动子在根癌土壤杆菌 (Agrobacteriumtumefaciens (SmithetTownsend)Conn)LBA4 4 0 4中的活性 ,研究发现在根癌土壤杆菌中CLCuV双向启动子的互补链启动子活性高于病毒链启动子 ,其在土壤杆菌中驱动的GUS活性为CaMV 3 5S启动子驱动的GUS活性的 2倍。同时 ,通过对一系列CLCuV双向启动子的互补链 5′端缺失体在土壤杆菌中的活性分析表明 - 2 87bp上游可能存在一负调控元件 ,该元件的缺失可使启动子活性达全长启动子的 4倍之多。还讨论了CLCuV互补链启动子所包含的其他顺式元件的功能。
Cotton leaf curl virus (CLCuV) is a single-stranded DNA virus belonging to the geminivirus subgroup III. The activity of the biVirus bidirectional promoter in Agrobacterium tumefaciens (SmitheTownsend) Conn LBA4404 was examined and it was found that the promoter activity of the CLCuV bidirectional promoter in Agrobacterium tumefaciens was higher than that of the viral chain , Which drives GUS activity in Agrobacterium twice as much as the GUS activity driven by the CaMV 35S promoter. At the same time, a series of CLCuV two-way promoter complementary strand 5 ’deletions in Agrobacterium tumefaciens activity analysis showed -287bp upstream may exist a negative regulatory element, the lack of this element can make the promoter activity of full-length start Four times as many children. The function of other cis-elements contained in the CLCuV complementary strand promoter is also discussed.