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目的:探讨补充3周丙酮酸钙对于小鼠力竭后组织损伤的保护作用机制。方法:雄性小鼠40只,随机分为安静对照组(C组)、运动对照组(Ec组)、丙酮酸钙组(P组)、运动丙酮酸钙组(Ep组)各10只,C及Ec组小鼠每天灌服10%阿拉伯胶溶液,P及Ep组每天给予600mg/kg的丙酮酸钙灌胃,共3周。3周末时各组大鼠均进行力竭运动实验,检测肝脏组织中过氧化氢(H2O2)和谷胱甘肽(GSH)的含量、ATP酶活力。结果:与Ec组比较,Ep组小鼠运动至力竭时间明显延长,运动能力显著提高(106.9±24.7min、167.0±31.2min,P<0.01)。Ep组GSH含量明显高于其它各组(P<0.05)。运动力竭后Ec组和Ep组H2O2含量明显高于C、P组,Ep组低于Ec组(P<0.01)。结论:补充丙酮酸钙能明显提高小鼠力竭运动能力和抗氧化损伤能力,其可能原因是提高GSH抗氧化能力及抵抗部分ATP酶的失活。
Objective: To investigate the mechanism of protective effect of calcium pyruvate for 3 weeks on tissue damage after exhaustion in mice. Methods: Forty male mice were randomly divided into 4 groups: control group (Ec group), exercise group (P group), exercise group (Ep group) And Ec mice were inoculated with 10% gum arabic solution daily, while P and Ep groups were administered with 600 mg / kg calcium pyruvate orally for 3 weeks. At the end of the third week, rats in each group were subjected to exhaustive exercise, and the content of hydrogen peroxide (H2O2) and glutathione (GSH) and the activity of ATPase in the liver tissues were detected. Results: Compared with Ec group, the mice in Ep group showed prolonged exercise to prolonged exhaustion and increased exercise capacity (106.9 ± 24.7 min, 167.0 ± 31.2 min, P <0.01). The content of GSH in Ep group was significantly higher than that in other groups (P <0.05). The content of H2O2 in Ec group and Ep group was significantly higher than that in C and P groups after Exhaustive exercise, while Ep group was lower than Ec group (P <0.01). CONCLUSION: Calcium pyruvate supplementation can significantly improve the exhaustive exercise capacity and anti-oxidative damage ability of mice, which may be due to the enhancement of GSH antioxidant capacity and resistance to partial ATPase inactivation.