目的探索骨髓间充质干细胞(Bone Marrow Mesenchymal Stem Cells,BMMSCs)和干细胞因子(Stem Cell Factor,SCF)促进肠黏膜细胞分化增殖和再生的机制,提供治疗老年炎症性肠病的认识。方法 (1)建立动物模型:由吲哚美辛诱导的胃肠黏膜损害,供体雄性SD老年大鼠皮下注射吲哚美辛处理(7.5mg/kg在5%碳酸氢钠)24小时连续3天,取4只吲哚美辛处理的SD老年大鼠使用戊巴比妥麻醉条件下处死(50mg/kg,腹腔注射)细胞组织学评估和确认老年炎症性肠病模型成功建立。(2)成功建立的老年炎症性肠病模型动物被分成4组,每组16只:BMMSCs-SCF治疗组,BMMSCs治疗组,SCF治疗组和生理盐水对照组。(3)使用PKH26荧光染色标记骨髓间充质基质细胞,输入炎症性肠病受试SD老年大鼠实验模型中,通过形态学观察测定肠黏膜的修复及再生,采用Western blot和PCR技术对动物模型体内骨髓间充质基质细胞中PKH26的表达情况。结果在受体老鼠胃肠黏膜中发现PKH26荧光染色标记的骨髓间充质基质细胞,如增殖细胞核抗原(proliferating cell nuclear antigen,PCNA),干细胞标记物(Lgr5,Musashi-1,ephrin-B3)等。在BMMSCs治疗组的SD老年大鼠肠黏膜中mRNA,PCNA的蛋白水平,Lgr5,Musashi-1和ephrin-B3增高,在BMMSCs-SCF混合治疗组中显著提高。在BMMSCs治疗组肠黏膜层和隐窝层浓度较高,更明显的在BMMSCs-SCF混合治疗组。结论在吲哚美辛诱导的肠道损伤实验中BMMSCs和SCF可能参与黏膜细胞再生并发挥协同作用。BMMSCs和SCF可能具有治疗价值。 Objective To explore the mechanism of bone marrow mesenchymal stem cells (BMSCs) and stem cell factor (Stem Cell Factor) in promoting the proliferation, proliferation and regeneration of intestinal mucosal cells, and to provide a basis for the treatment of senile inflammatory bowel disease. Methods (1) Animal models were established: Indomethacin-induced gastrointestinal mucosal lesion, male SD male rats were injected subcutaneously with indometacin (7.5mg / kg in 5% sodium bicarbonate) for 24 hours continuously Day, 4 indometacin-treated SD rats were sacrificed by pentobarbital anesthesia (50mg / kg, intraperitoneal injection) for histological evaluation and confirmation of the establishment of the model of senile inflammatory bowel disease. (2) The model of senile inflammatory bowel disease successfully established animals were divided into four groups of 16: BMMSCs-SCF treatment group, BMMSCs treatment group, SCF treatment group and saline control group. (3) Marrow mesenchymal stromal cells were labeled with PKH26 fluorescent staining, and then enter into the experimental model of inflammatory bowel disease SD rats. Morphological observation was used to detect the repair and regeneration of intestinal mucosa. Model of bone marrow mesenchymal stromal cells PKH26 expression. Results PKH26 fluorescent-labeled bone marrow mesenchymal stromal cells (PCNA), stem cell markers (Lgr5, Musashi-1, ephrin-B3) were found in the gastrointestinal mucosa of recipient mice . The levels of mRNA and protein of PCNA, Lgr5, Musashi-1 and ephrin-B3 in the intestinal mucosa of BMMSCs-treated SD rats were significantly increased in BMMSCs-SCF mixed treatment group. Intestinal mucosa and crypt layers were higher in the BMMSCs treated group, more significantly in the BMMSCs-SCF mixed treatment group. Conclusion BMMSCs and SCF may participate in mucosal cell regeneration and play synergistic effects in indomethacin-induced intestinal injury. BMMSCs and SCF may have therapeutic value.