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目的 探讨二乙基二硫代氨基甲酸钠 (DDC)对耐药胃癌细胞的作用及可能的机理。方法 应用MTT方法 ,化学发光法测定SOD活性以及流式细胞仪检测细胞内药物浓度。结果 DDC与阿霉素 (adriamycin ,adr)共同应用可明显抑制耐药细胞及药敏肿瘤细胞的生长。在DDC组 ,adr对SGC790 1/WT的半数生长的抑制剂量 (IC5 0 )为 0 79μg/mL。在同一组中 ,adr对SGC790 1/VCR的IC5 0为 0 3 2 μg/mL。流式细胞术证实 ,耐药细胞中的药物蓄积量为药敏细胞中的1/2 ,SOD活性检测表明 ,DDC明显抑制了SOD活性。结论 超氧化物歧化酶在耐药细胞中的变化对肿瘤的治疗具有重要的意义
Objective To investigate the effect and possible mechanism of diethyldithiocarbamate (DDC) on drug-resistant gastric cancer cells. Methods MTT assay, chemiluminescence determination of SOD activity and flow cytometry intracellular drug concentration. Results DDC combined with adriamycin (adr) could obviously inhibit the growth of drug-resistant and drug-resistant tumor cells. In the DDC group, the inhibitory dose of adr to half-growth of SGC790 1 / WT (IC 50) was 0 79 μg / mL. In the same group, the IC50 of adr for SGC790 1 / VCR was 032 μg / mL. Flow cytometry confirmed that drug-resistant cells in the drug accumulation of drug-sensitive cells in 1/2, SOD activity test showed that DDC significantly inhibited the activity of SOD. Conclusion The change of superoxide dismutase in drug-resistant cells is of great significance for the treatment of cancer