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目的体外诱导培养正常儿童与B系急性淋巴细胞白血病(B-ALL)儿童的树突状细胞(DC),比较二者的生物学特性。方法分离10例B-ALL初诊患儿(ALL组)和10例正常儿童(对照组)的外周血单个核细胞,以rh GM-CSF(20 ng/m L)、rh IL-4(10 ng/m L)及TNF-α(10 ng/m L)联合培养8 d,显微镜下观察细胞形态,流式细胞仪检测细胞免疫表型(CD11c、CD80、CD83、CD86),ELISA检测培养上清中IL-12的浓度,混合淋巴细胞反应(MLR)检测抗原递呈功能,电化学法检测上清中葡萄糖浓度。结果 ALL组细胞未表现出DC的典型形态,CD11c、CD80、CD83、CD86表达均较对照组细胞低(P<0.05),分泌IL-12的能力弱于对照组细胞(P<0.05),刺激T淋巴细胞增殖的能力弱于对照组细胞,ALL组细胞培养上清中的葡萄糖浓度高于对照组(P<0.05)。结论B-ALL来源的DC成熟度异常,功能减弱,葡萄糖代谢异常可能是其成熟异常的原因之一。
OBJECTIVE: To induce the dendritic cells (DCs) from normal children and B children with acute lymphoblastic leukemia (B-ALL) in vitro and to compare their biological characteristics. Methods Peripheral blood mononuclear cells of 10 newly diagnosed B-ALL children (ALL group) and 10 normal children (control group) were isolated and cultured with rhGM-CSF (20 ng / m L), rhIL-4 / ml and TNF-α (10 ng / ml) for 8 days. The cell morphology was observed under a microscope. The cell immunophenotypes (CD11c, CD80, CD83, CD86) were detected by flow cytometry. IL-12, mixed lymphocyte reaction (MLR) were used to detect the antigen presenting function, and the concentration of glucose in the supernatant was detected by electrochemical method. Results The expression of CD11c, CD80, CD83 and CD86 in ALL group was lower than that in control group (P <0.05), IL-12 secretion was weaker than that in control group (P <0.05) The ability of T lymphocyte proliferation was weaker than that of control group. The concentration of glucose in cell culture supernatant of ALL group was higher than that of control group (P <0.05). Conclusion B-ALL-derived DCs may be one of the causes of abnormal maturation of DC with abnormal function, weakened function and abnormal glucose metabolism.