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本文报道用大片段缩合法合成DNA的新方法。该方法的原理是:用固相亚磷酰胺方法合成DNA片段,以氢氧化钠的乙醇、水混合溶液选择性地水解连于CPG载体与
This paper reports a new method of DNA synthesis using large fragment condensation. The principle of the method is as follows: the DNA fragments are synthesized by the method of solid-phase phosphoramidite, the mixed solution of sodium hydroxide and ethanol is used to selectively hydrolyze the DNA fragments linked to the CPG carrier and