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供试材料为52份冬小麦品种。以其中7份先行干种子30KRγ射线照射处理,然后从M_1成株中取未成熟胚进行离体培养,M_1的平均出愈率比对照提高40%,分化率保持不动,分化历期延长10天,得苗率显著增高。对4份材料的成株分别在原胚期及幼胚期进行1KR照射处理,取未成熟胚离体培养,原胚期的出愈率比对照成倍提高,分化率却降低一半,得苗率随之下降;幼胚期的出愈率不变,分化率提高,得苗率增加。用12份材料的愈伤组织进行1KR照射处理,分化率显著下降。其它29份材料用5-100KR不同照射量处理,发现5KR是抑制分化的照射量,25KR是抑制生长的起始照射量。
The test material for 52 winter wheat varieties. Seven of them were treated with 30KR γ-ray irradiation, and then immature embryos were taken from M_1 adult plants for in vitro culture. The average recovery rate of M_1 was 40% higher than that of the control, the differentiation rate remained unchanged and the differentiation period prolonged 10 Days, seedling rate was significantly higher. The adult plants of 4 materials were treated with 1KR in the primordial and early embryos respectively. The immature embryos were ex-vivo cultured. The embryogenic calluses in the 4 embryos were doubled and the differentiation rate was reduced by half. With the decline; the immature embryo out of the same rate of change, increased differentiation rate, increased seedling rate. With 12 material callus 1KR irradiation treatment, the differentiation rate decreased significantly. The other 29 materials treated with different doses of 5-100KR, found that 5KR is the irradiation dose to inhibit differentiation, 25KR is the initial dose to inhibit growth.