本文利用系数倍率法消除盐酸小蘖碱、黄芩甙、大黄浸膏及辅料紫外吸收的相互干扰,从而不经分离同时分别测定三黄片中盐酸小蘖碱、黄芩甙的含量。测定盐酸小蘖碱的波长对为267nm,291nm和k值为1.409;黄芩甙的波长对为277nm,250nm和k值为1.136。盐酸小蘖碱、黄芩甙的平均回收率分别为;98.62%—98.37%;变异系数分别为:2.75%、1.36%(n=6) In this paper, coefficient of multiplication method to eliminate the interference of berberine hydrochloride, baicalin, rhubarb extract and excipients UV absorption, without separation were determined at the same time berberine hydrochloride berberine, baicalin content. The wavelength of berberine hydrochloride was determined to be 267 nm, 291 nm and k value of 1.409; the wavelength pair of baicalin was 277 nm, the wavelength of 250 nm and the value of k were 1.136. The average recoveries of berberine hydrochloride and baicalin were 98.62% -98.37%, and the coefficients of variation were 2.75% and 1.36% (n = 6)