目的　探讨沙眼衣原体 (CT)DNA在输卵管性不孕患者输卵管和宫颈表达的特点。方法　 2 0 0 1年 5月至 2 0 0 2年 2月 ,中山大学附属第二医院采用荧光定量PCR检测 4 3例输卵管性不孕患者 (病例组 )和 2 6例因其他良性疾病而同时切除输卵管且术中未发现输卵管病变患者 (对照组 )的宫颈分泌物和输卵管黏膜CTDNA。结果　病例组宫颈和输卵管CTDNA阳性率分别为 2 5 6 %、33 3% ,明显高于对照组 (P <0 0 5 )。病例组宫颈和输卵管CTDNA的定量值高于对照组 (P <0 0 5 )。宫颈与输卵管CTDNA表达的相关系数为 0 883(P <0 0 5 ) ,一致率高达 94 5 % (P >0 0 5 )。结论　CT感染与输卵管性不孕症密切相关 ;宫颈分泌物CTDNA检查能较好地反映输卵管CT感染。 Objective To investigate the expression of Chlamydia Trachomatis (CT) DNA in the fallopian tubes and cervix in patients with tubal infertility. Methods From May 2001 to February 2002, the Second Affiliated Hospital of Sun Yat-sen University detected 43 cases of tubal infertility (case group) and 26 cases of other benign diseases by fluorescence quantitative PCR Cervical secretions and tubal mucosa CT DNA were removed from the fallopian tubes without tubal lesions (control group). Results The positive rates of CTDNA in cervix and fallopian tube were 25.6% and 33.3% respectively, which were significantly higher than those in control group (P <0.05). The quantitative value of CTDNA in cervical and tubal cases in case group was higher than that in control group (P <0.05). The correlation coefficient of cervical and tubal CT DNA expression was 0 883 (P 0 05), the coincidence rate was 94 5% (P 0 05). Conclusion CT infection is closely related to tubal infertility; CTDNA examination of cervical secretions can better reflect tubal CT infection.