论文部分内容阅读
目的探讨中波紫外线(UVB)对人永生化表皮(Ha Ca T)细胞的损伤作用,为紫外线损伤防护提供技术支持。方法取体外培养Ha Ca T细胞,用0(对照组)、10、30、60 m J/cm2剂量的UVB照射后继续培养12 h,以流式细胞术检测细胞凋亡率,用彗星实验(单细胞凝胶电泳)检测细胞DNA损伤情况。结果对照组、10、30、60 m J/cm2紫外线照射组Ha Ca T细胞早期凋亡率分别为(1.2±0.14)%、(2.23±0.37)%、(4.13±0.29)%、(5.97±0.17)%,晚期凋亡率分别为(2±0.13)%、(7.63±0.52)%、(23.36±0.98)%、(40.46±1.39)%,与对照组比较,UVB照射组Ha Ca T细胞早期、晚期凋亡率明显增加,差异有统计学意义(P<0.05);对照组、10、30、60 m J/cm2紫外线照射组Ha Ca T细胞DNA尾矩分别为(0.13±0.25)、(21.78±2.31)、(53.48±5.66)、(79.16±7.47),Olive尾矩分别为(0.32±0.47)、(16.17±1.96)、(36.43±2.89)、(51.71±1.87),与对照组比较,UVB照射组Ha Ca T细胞DNA尾矩、Olive尾矩明显延长,差异有统计学意义(P<0.05)。结论 UVB照射可使Ha Ca T细胞DNA产生损伤,诱发细胞凋亡,细胞DNA损伤可能是某些高原罕见病的发病原因之一。
Objective To investigate the effect of UVB on the injury of human immortalized epidermis (HaCa T) cells and provide technical support for the protection of UV rays. Methods HaCa T cells were cultured in vitro and irradiated with UVB (0, 30, 30, 60 mJ / cm2) for 12 h. The apoptosis rate was determined by flow cytometry. Single cell gel electrophoresis) to detect DNA damage. Results The apoptotic rate of HaCa T cells in control group was (1.2 ± 0.14)%, (2.23 ± 0.37)%, (4.13 ± 0.29)%, (5.97 ± 0.17%, respectively. The rates of apoptosis were (2 ± 0.13)%, (7.63 ± 0.52)%, (23.36 ± 0.98)%, (40.46 ± 1.39)% respectively. Compared with the control group, HaCa T cells The apoptosis rate of HaCa T cells in 10, 30 and 60 mJ / cm2 UV irradiation group was (0.13 ± 0.25) days, respectively, and the difference was statistically significant (P <0.05) (21.78 ± 2.31), (53.48 ± 5.66), (79.16 ± 7.47) and Olive tail moments were (0.32 ± 0.47), (16.17 ± 1.96), (36.43 ± 2.89) and (51.71 ± 1.87) In comparison, DNA tail moment and Olive tail moment of HaCa T cells in UVB irradiation group were significantly prolonged (P <0.05). Conclusion UVB irradiation can cause DNA damage in HaCa T cells and induce cell apoptosis. DNA damage may be one of the pathogenesis of rare high altitude disease.