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为了观察冷冻保存对肝癌患者粘附性LAK (A LAK )细胞生物学特性的影响。我们采用苯丙氨酸甲酯 (PME )处理肝癌患者外周血单个核细胞 (PBMC )以制备A LAK细胞 ,并观察冷冻保存后A LAK细胞增殖能力、杀伤活性及细胞表型的变化。实验结果显示 ,A LAK细胞在冷冻复苏后的短时间内 ,增殖能力、杀伤活性均受到损伤 ,但随着IL 2的刺激而迅速恢复。至复苏后第 5天 ,冻存的A LAK细胞的杀伤活性明显高于未经冻存的新鲜A LAK细胞。而A LAK细胞的表面标志则在冷冻前后无显著性改变。因此 ,冷冻保存可促进肝癌患者A LAK细胞的免疫活性
To observe the effect of cryopreservation on the biological characteristics of adhesive LAK (A LAK) cells in patients with liver cancer. We used phenylalanine methyl ester (PME) to treat peripheral blood mononuclear cells (PBMCs) of patients with hepatocellular carcinoma to prepare A LAK cells. The proliferation, cytotoxicity and cell phenotype of A LAK cells after cryopreservation were observed. The results showed that the proliferation and cytotoxicity of A LAK cells were damaged in a short period of time after cryopreservation, but rapidly recovered with the stimulation of IL 2. By day 5 after resuscitation, the cytotoxic activity of frozen A LAK cells was significantly higher than that of fresh A LAK cells without freezing. However, the surface markers of A LAK cells did not change significantly before and after freezing. Therefore, cryopreservation can promote the immunological activity of A LAK cells in liver cancer patients