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目的 探讨锰的雄性生殖毒性及对氨基水杨酸钠 (PAS Na)的治疗作用机理。方法 亚慢性染毒法给雄性大鼠染毒 (MnCl2 ·4H2 O每天 15mg/kg ,ip) 12周 ,然后 ,用PAS Na每天 12 0mg/kg ,ip ,(用药 3d ,停 4d) 3周 ,观察锰对睾丸细胞内钙离子调节酶的影响及PAS Na对其的干预作用。结果 染锰可使睾丸细胞的质膜、线粒体、微粒体内的Ca2 + Mg2 + ATPase、Ca2 + ATPase活性及巯基含量下降 ,其中以质膜和线粒体最为明显 (P <0 0 5 ) ,同时 ,质膜上Na+ K+ ATPase活性也显著受抑 (P <0 0 1)。PAS Na对上述三个亚细胞组分中受抑的Ca2 + Mg2 + ATPase、Ca2 + ATPase及下降的巯基含量均有促其恢复至正常的作用 ,使质膜上的Na+ K+ ATPase活性也有所恢复。结论 PAS Na对锰所致的睾丸细胞内钙稳态失调有一定的恢复作用
Objective To investigate the male reproductive toxicity of manganese and its mechanism of action on sodium salicylate (PAS Na). Methods Male rats were exposed to sub-chronic exposure (MnCl 2 · 4H 2 O 15 mg / kg, ip) for 12 weeks, then treated with PAS Na 120 mg / kg ip daily for 3 days To observe the effect of manganese on intracellular calcium ion-regulating enzyme in testis and its effect on PAS Na. Results Manganese exposure could decrease the activities of Ca2 + Mg2 + ATPase, Ca2 + ATPase and the content of sulfhydryl in the plasma membrane, mitochondria and microspheres of testis cells, the most obvious was plasma membrane and mitochondria (P <0.05) Membrane Na + K + ATPase activity was also significantly inhibited (P <0.01). PAS Na repressed Ca2 + Mg2 + ATPase, Ca2 + ATPase and decreased sulfhydryl content in the above three subcellular components, which promoted the normalization of Na + K + ATPase activity on the plasma membrane . Conclusion PAS Na can restrain manganese homeostasis in testis cells