【摘 要】
：
The effects of various Ca2+-modifying drugs on moue egg fertilization were studied. Ca2+ chelator,rnethylen glycol-bis-(2-aminoethyl)-tetracetic acid (EGTA) ,an
【机 构】
：
State Key Laboratory of Reproductive Biology,Institute of Zoology,Academia Sinica,Beijing 100080 PRC
论文部分内容阅读
The effects of various Ca2+-modifying drugs on moue egg fertilization were studied. Ca2+ chelator,rnethylen glycol-bis-(2-aminoethyl)-tetracetic acid (EGTA) ,and calmodulin (CaM) antagonist,trifluoperzainern(TFP) ,inhibited fertilization in a dose-dependent manner,whild Ca2+ channel bolcker,verapamil ,did not havernany effect. When intracellular Ca2+ release was blocked by 8-(N, N-diethylamino) octy1-3,4,5-trimethoxy-rnbenzonate (TMB-8) or the Ca2+ oscillations were inhibited by an inhibitor of endoplasmic reticulum Ca2+-AT-rnPase,thapsigargin,the second polar body emission and pronuclear formation were significantly decreased. Inrncontrast,inhibition of intracellular Ca2+ release via bolckage of inositol 1,4,5-triphosphate (IP3) production by neomycin or lithium did not affect fertilization. The results sugest that both extracellular influx,intracellu-rnlar Ca2+ release and CaM activation are required for normal fertilization. However ,extracellular influx throughrnvoltage-gated Ca2+ channel and intracellular release induced by IP3 are not the only pathways for producingrnCa2+ transients in moue eggs.
其他文献
北沙参别名珊瑚菜.主产于我国北部.多年生草木.株高30厘米,主根细长,圆柱形.长约40厘米.茎大部分埋在沙土中,部分露出地面,密被灰绿色绒毛,不分枝.喜温暖湿润的气候.以肥沃的
慢性食管炎为消化系统常见病.多有胸内闷痛病史,且疼痛可因进食过热、粗糙、干燥而加重.部分患者进食后不久,即呕吐无酸味的食物.此病少见吞咽困难.有与吞咽有关的胸背部疼痛
Linked tetra-shaft and double-cantilever flat flap gate is a new type of structure in water conservancyrnprojects ,but the traditional method is now adopted in
Soybean is generally recognized as a source of edible and the deoiled meal is seen as a source of protein inrnanimal feed. In recent years. However,more interes
哈密瓜东移栽培试验表明,在北京周边栽培哈密瓜非常容易.对土质要求不严,pH值5～9,含盐量在1%以下,腐植质在1.5%以上的沙壤土最好;温度在28～38℃,相对湿度在15%～70%,光照3万勒克
留置导尿是一项无菌操作技术,也是临床常见的护理技术操作,而由留置导尿引发的尿路感染也是临床常见的疾病,在我国医院感染中仅次于呼吸道感染.因此,在留置导尿过程中为了避
During the design of pipeline,the determination of local resistant coefficient is often come arcoss. Thernsudden enlargement local resistant coefficient ζ1 = [
结合森林公安系统委托办理的偷盗古树案件，以涉案的3株海螺望春花（Magnolia denudate cv．Hai－luo）为对象，根据其特征性木材构造之轴向薄壁组织进行生长轮界定，通过生长轮宽度测试及
目的 探讨先天性唇腭裂治疗过程中患儿的术后专科护理.方法 395例唇腭裂在气管全麻下进行唇腭裂修补术,唇裂采用弧形修补法或改良MILLARD法,腭裂采用兰氏修复法或双瓣修补法,
The even degree of animal population is generlay measured by the coefficient of variation of major eco-rnnomic characters. Facing the coefficient of variation,a