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目的 :探讨喉咽鳞状细胞癌 (LPSCC)D9S171微卫星位点杂合性缺失 (LOH)的临床意义及对检测颈淋巴结微转移的应用价值。方法 :应用聚合酶链反应对 18例LPSCC及 72个颈淋巴结在 9号染色体上的D9S171微卫星位点进行扩增 ,分析D9S171微卫星位点的DNA等位基因的LOH。结果 :颈淋巴结组织D9S171位点的LOH发生率 4 0 .2 8%显著高于常规病检的阳性率 8.33% (P <0 .0 1) ;肿瘤组织中的LOH发生率与肿瘤分化程度有关 (P <0 .0 5 ) ,并且与肿瘤临床分期呈密切线性正相关性 (P <0 .0 1)。结论 :D9S171位点的LOH分析可能是检测LPSCC颈淋巴结微转移的较为敏感的手段之一 ,并可能作为判断LPSCC患者预后的指标之一。
Objective: To investigate the clinical significance of heterozygosity deletion (LOH) of microsatellite locus D9S171 in laryngeal squamous cell carcinoma (LPSCC) and its clinical value in detection of micrometastasis in cervical lymph nodes. Methods: Polymerase chain reaction (PCR) was used to amplify the D9S171 microsatellite locus on chromosome 9 in 18 cases of LPSCC and 72 cervical lymph nodes. The LOH of DNA allele of D9S171 microsatellite locus was analyzed. Results: The incidence of LOH in D9S171 cervical lymph node tissue was 40.28%, which was significantly higher than that in routine pathology (8.33%, P <0.01). The incidence of LOH in tumor tissue correlated with the degree of tumor differentiation (P <0.05), and was positively correlated with the clinical stage of tumor (P <0.01). Conclusion: The LOH analysis of D9S171 locus may be one of the more sensitive means to detect micrometastasis of cervical lymph nodes in LPSCC and may be used as one of the indicators to judge the prognosis of LPSCC patients.