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目的:观察牙龈卟啉单胞菌脂多糖(Pg-lps)对体外培养大鼠血管平滑肌细胞(vascular smooth muscle cells,VSMCs)增殖和碱性磷酸酶(ALP)活性的影响。方法:采用组织块贴壁法培养大鼠VSMCs,传代培养并鉴定。Pg-lps(1~10000 ng/mL)干预VSMCs 0.5~4 d,采用CCK-8法和碱性磷酸酶试剂盒测定其增殖和ALP活性。结果:1 d后(1-10000 ng/mL)Pg-lps均显著促进VSMCs增殖,并随时间的延长,促进作用越明显;而(1 ng/mL、1μg/mL)Pg-lps在0.5~4 d均显著促进VSMCs的ALP活性,随着时间的延长促进作用更明显,且4 d时1μg/mL较1 ng/mL对ALP活性促进作用更明显。结论:Pg-lps能促进VSMCs的异常增殖;还可能通过促进VSMCs的ALP活性而影响其钙化过程,进而提示Pg-lps对心血管疾病的发生发展可能有重要作用。
Objective: To observe the effects of Pg-lps on the proliferation and alkaline phosphatase (ALP) activity of cultured vascular smooth muscle cells (VSMCs) in vitro. Methods: The rat VSMCs were cultured by tissue block adhesion method, subcultured and identified. VSMCs were treated with Pg-lps (1 ~ 10000 ng / mL) for 0.5 ~ 4 days. The proliferation and ALP activity of VSMCs were determined by CCK-8 assay and alkaline phosphatase kit. Results: Pg-lps (1-10000 ng / mL) significantly promoted the proliferation of VSMCs after 1 day, and with the prolongation of time, the more obvious the promotion effect was. Pg-lps of 1 ng / mL and 1 g / 4 d significantly promoted the ALP activity of VSMCs. The effect was more obvious with the prolongation of time. The effect of 1 μg / mL 1 ng / mL on ALP activity was more obvious at 4 d. Conclusion: Pg-lps can promote the abnormal proliferation of VSMCs, and may also affect the calcification process by promoting the ALP activity of VSMCs, suggesting that Pg-lps may play an important role in the development of cardiovascular diseases.