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目的 探讨免疫球蛋白基因SNC73在不同恶性肿瘤组织及其自身对照正常组织中的表达及临床意义。方法 采用逆转录聚合酶链反应酶联免疫吸附 (RT PCR ELISA)法 ,检测SNC73基因在 90例恶性肿瘤 (大肠癌、胃癌、乳腺癌、肺癌、肝癌 )及其相对应的对照正常组织中的表达 ,并对SNC73表达与大肠癌患者的性别、年龄、发病部位、分化程度、侵袭深度和转移等临床病理特征的相关性进行分析。结果 SNC73基因在正常黏膜及大肠癌表达量的比值为 7.134 ,差异有显著性 (P <0 .0 1)。胃癌、乳腺癌、肺癌和肝癌组织的SNC73表达量与自身对照正常组织比较 ,差异无显著性(P >0 .0 5 )。结论 SNC73的表达下调是大肠癌相对特异的现象 ,提示其可能为大肠癌发生发展中新的肿瘤标记 ,其与大肠癌发生发展的相关性 ,值得进一步研究。
Objective To investigate the expression and clinical significance of immunoglobulin SNC73 in different malignant tumor tissues and their own control normal tissues. Methods Reverse transcription polymerase chain reaction (RT PCR) was used to detect SNC73 in 90 malignant tumors (colorectal cancer, gastric cancer, breast cancer, lung cancer and liver cancer) and their corresponding control normal tissues The correlation between the expression of SNC73 and clinicopathological features such as gender, age, site of disease, differentiation, invasion depth and metastasis of colorectal cancer were analyzed. Results The ratio of SNC73 gene expression in normal mucosa and colorectal cancer was 7.134, the difference was significant (P <0.01). The expression of SNC73 in gastric cancer, breast cancer, lung cancer and hepatocellular carcinoma tissues was not significantly different from the normal control tissues (P> 0.05). Conclusion The down-regulation of SNC73 expression is a relatively specific phenomenon in colorectal cancer, suggesting that it may be a new tumor marker in the development of colorectal cancer. It is worthy of further study on the relationship between SNC73 expression and the occurrence and development of colorectal cancer.