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为了解决微流控电泳芯片集成化问题,设计并制作出一种具有管道两侧微阵列电极结构的硅-PDMS复合低电压电泳芯片.通过电路控制程序在微侧壁阵列电极上施加交替循环的低电压,以实现芯片微管道中低电压电泳过程;并对硅-PDMS芯片的电绝缘性、伏安曲线及电渗流等性能进行了测试和评价.以pH为10.0、10mmol/L的硼砂作为缓冲体系,分离场强150V/cm、切换时间3s的条件下,完成了10-4mol/L的苯丙氨酸和精氨酸的低电压电泳分离,分离度达1.6,实现了两种氨基酸的完全分离.在此基础上,将系统用于牛血清白蛋白和α-乳白蛋白的分离,并初步实现了该两种蛋白质的芯片电泳分离.
In order to solve the integration problem of microfluidic electrophoresis chip, a silicon-PDMS composite low-voltage electrophoresis chip with micro-array electrode structure on both sides of the pipeline was designed and fabricated.An alternating cycle Low voltage in order to achieve the low-voltage electrophoresis process in the micro-pipeline of the chip, and the electrical insulation, volt-ampere curve and electroosmotic flow performance of the silicon-PDMS chip were tested and evaluated.With borax pH 10.0 and 10mmol / L as Buffer system, the separation field strength of 150V / cm, the switching time of 3s, the completion of 10-4mol / L phenylalanine and arginine low voltage electrophoresis separation, resolution of 1.6, to achieve the two amino acids Completely separated.On this basis, the system was used for the separation of bovine serum albumin and α-lactalbumin, and the chip electrophoresis separation of the two proteins was initially achieved.