论文部分内容阅读
目的探讨基质金属蛋白酶(MMP-9)及其组织抑制物(TIMP-1)mRNA与卵巢上皮性癌的发生、发展的关系。方法采用RT-PCR技术检测69例卵巢上皮性肿瘤(38例恶性、15例交界性、16例良性)及11例正常卵巢组织中MMP-9及TIMP-1mRNA的表达,并分析其与各临床病理特征的关系。结果MMP-9mRNA在卵巢上皮性癌及交界性肿瘤组织中的阳性表达率(分别为76%、44%)及表达水平[分别为(154.14±4.42)、(96.87±3.04)]均显著高于良性肿瘤及正常卵巢组织[分别为13%、9%和(8.26±2.67)、(2.34±1.02);P<0.05];且MMP-9mRNA在晚期卵巢上皮性癌组织中的阳性表达率和表达水平明显高于早期癌组织(P<0.05)。TIMP-1mRNA在卵巢上皮性癌及交界性肿瘤组织中的阳性表达率(分别为68%、38%)及表达水平[分别为(126.35±3.81)、(90.62±5.54)]均显著高于良性肿瘤及正常卵巢组织[分别为13%、9%和(8.32±2.02)、(2.63±1.35);P<0.05];TIMP-1mRNA在卵巢上皮性癌组织中的阳性表达率和表达水平与各临床病理特征均无相关性(P>0.05)。结论MMP-9mRNA的高表达及MMP-9/TIMP-1平衡的调节失衡导致卵巢上皮性肿瘤的发生、侵袭和转移;MMP-9mRNA的高表达对卵巢上皮性癌有预后意义。
Objective To investigate the relationship between the expression of matrix metalloproteinase (MMP-9) and tissue inhibitor of metalloproteinase (TIMP-1) and the occurrence and development of epithelial ovarian cancer. Methods RT-PCR was used to detect the mRNA expression of MMP-9 and TIMP-1 in 69 cases of ovarian epithelial tumor (38 cases of malignant, 15 cases of borderline, 16 cases of benign) and 11 cases of normal ovarian tissue. Pathological characteristics of the relationship. Results The positive expression rates of MMP-9 mRNA in epithelial ovarian cancer and borderline tumor tissues were 76%, 44% and 154.14 ± 4.42, respectively (96.87 ± 3.04) (13%, 9%, and (8.26 ± 2.67), (2.34 ± 1.02), respectively; P <0.05]; and the positive rate and expression of MMP-9 mRNA in advanced epithelial ovarian cancer The level was significantly higher than that of early stage cancer (P <0.05). The positive expression rates of TIMP-1 mRNA in ovarian epithelial carcinoma and borderline tumor tissues (68%, 38%, respectively) and their expression levels (126.35 ± 3.81, 90.62 ± 5.54, respectively) were significantly higher than those in benign (13%, 9%, and (8.32 ± 2.02), (2.63 ± 1.35), respectively; P <0.05]. The positive rates and expression levels of TIMP-1 mRNA in epithelial ovarian cancer tissues were significantly different from those in normal ovarian tissues There was no correlation between clinical and pathological features (P> 0.05). Conclusions The imbalanced regulation of MMP-9 mRNA and MMP-9 / TIMP-1 balance leads to the occurrence, invasion and metastasis of ovarian epithelial tumor. The high expression of MMP-9 mRNA has prognostic significance for epithelial ovarian cancer.