蛋白质是一类重要的生物大分子,其结构与性质得到了广泛的研究.差示扫描微量热法(DSC)可以获得蛋白质的热容(C_p)与温度的关系,对我们认识蛋白质的性质有重要的帮助作用.同时,DSC研究蛋白质相关体系新方法也在不断涌现.本文介绍了差示扫描微量热法在研究蛋白质解折叠机理、蛋白质稳定性以及复杂蛋白质体系等几个方面的进展. Proteins are an important class of biological macromolecules whose structures and properties have been extensively studied. The differential scanning calorimetry (DSC) can obtain the relationship between the protein’s heat capacity (C_p) and temperature, and we know the nature of the protein Important help.At the same time, new methods of DSC research on protein-related systems are also emerging.This paper introduces the progress of Differential Scanning Calorimetry in the study of protein unfolding mechanism, protein stability and complex protein system.