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采用AFLP技术分别对太湖、长江日本沼虾(Macrobrachium nipponense)群体各30尾的基因组DNA进行了群体特异性位点比对分析,并对各位点基因频率分布进行正态性检验、方差齐性检验(F检验)、样本平均数差异显著性检验(t检验)。结果显示:3对引物共扩增出138个位点;太湖组是独立于长江组的群体,而且它们是两个分化了的野生群体;采用变异性的显著性检验(χ2检验)对位点进行差异比较发现,23个位点在太湖组中出现的频率显著高于长江组,其中位点BY160、BY320、BY470、BY520是与太湖群体分化显著关联的位点,可作为区别太湖野生群体与长江野生群体的标记。
AFLP technique was used to analyze the genome-specific loci of 30 genomic DNA in each of the populations of Taihu Lake and Yangtze River Macrobrachium nipponense. The frequency distribution of each locus was normalized, homogeneity of variance test (F test), sample mean difference significant test (t test). The results showed that a total of 138 loci were amplified by 3 pairs of primers. The Taihu Lake group was independent of the Yangtze River group, and they were two differentiated wild populations. Using the variational significance test (χ2 test) The frequency of occurrence of the 23 loci in Taihu Lake was significantly higher than that of the Yangtze River. The loci BY160, BY320, BY470 and BY520 were significantly associated with the population differentiation of Taihu Lake and could be used as a marker to distinguish between wild populations in Taihu Lake and The Yangtze River wild group mark.