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目的研究楔叶茶藨地上部分的化学成分。方法体积分数95%乙醇回流提取楔叶茶藨,利用硅胶、Sephadex LH-20、半制备HPLC等多种色谱技术对其进行分离纯化,并根据化合物的波谱数据和理化性质进行结构鉴定。结果从楔叶茶藨体积分数95%乙醇提取物中分离并鉴定了19个化合物的结构,分别为槲皮素(1),槲皮素-3-O-β-D-葡萄糖苷(2),槲皮素-3-O-α-L-鼠李糖苷(3),槲皮素-3-O-β-D-新橙皮糖苷(4),mearnsetin(5),杨梅素-3-O-α-L-鼠李糖苷(6),杨梅素-3-O-β-D-葡萄糖苷(7),mearnsetin 3-O-β-D-glucopyranoside(8),mearnsetin 3-O-α-L-rhamnopyranoside(9),山柰酚-3-O-β-D-葡萄糖苷(10),山柰酚-3-O-(2-O-α-L-鼠李糖基)-β-D-葡萄糖苷(11),山柰酚-3-O-(2″,6″-二-O-α-L-鼠李糖基)-β-D-葡萄糖苷(12),1,2,4-苯三酚(13),香草酸(14),原儿茶酸(15),对羟基苯甲酸(16),没食子酸(17),blumenol C glucoside(18),conocarpan(19)。结论化合物1~19均为首次从该植物中分离得到,并且首次报道了化合物8的核磁数据。
Aim To study the chemical constituents in the aboveground parts of Schisandra chinensis. Methods The extract was fractionated by volume fraction of 95% ethanol and was separated and purified by silica gel, Sephadex LH-20, semi-preparative HPLC and other chromatographic techniques. The structures of the compounds were identified by spectral data and physical and chemical properties. Results The structures of 19 compounds were isolated and identified from 95% ethanolic extract of Quzhouia scoparium. They were quercetin (1), quercetin -3-O-β-D-glucoside , Quercetin -3-O-α-L-rhamnoside (3), quercetin -3-O-β-D-neohesperidin (4), mearnsetin (5), myricetin- (6), myricetin-3-O-β-D-glucoside (7), mearnsetin 3-O-β-D-glucopyranoside (8), mearnsetin 3-O-α -L-rhamnopyranoside (9), kaempferol-3-O-β-D-glucoside (10), and kaempferol-3-O- -D-glucoside (11), kaempferol-3-O- (2 “, 6” Vanillic acid (14), protocatechuic acid (15), p-hydroxybenzoic acid (16), gallic acid (17), blumenol C glucoside (18), conocarpan (19) . Conclusions Compounds 1 ~ 19 were isolated from this plant for the first time, and the NMR data of compound 8 were reported for the first time.